一种针对低浓度应用的高精度微型流式细胞仪  

A High-Accuracy Micro-Flow Cytometer for Low Concentration Applications

作　　者：王丽平 洪陵成[1,2] 郭天义 Wang Liping;Hong Lingcheng;Guo Tianyi(School of Environment,Hohai University,Nanjing 210098,China;Jiangsu Delin Environmental Protection Technologies Co.,Ltd.,Nanjing 211101,China)

机构地区：[1]河海大学环境学院,南京210098 [2]江苏德林环保技术有限公司,南京211101

出　　处：《微纳电子技术》2019年第2期133-139,共7页Micronanoelectronic Technology

摘　　要：微型流式细胞仪综合了流式细胞术和微流体的优势,已成为生物医学、临床应用领域低成本的细胞检测、分析方法。微型流式细胞仪的一个新兴应用是快速检测低浓度的细胞或粒子。基于一种集成了片内光束整形和微流体通道的微加工芯片,自主设计搭建了一种微型流式细胞仪。利用该微型流式细胞仪进行了实时微珠计数实验,实验结果表明微珠通量与样品的体积流量、样品浓度之间的线性相关系数均大于0.99。高线性结果验证了这种微型流式细胞仪的高精度计数功能,使其成为低浓度应用的理想选择,如检测食物或水中的病原体。The micro-flow cytometer,which combines the advantages of the flow cytometry and microfluidic,becomes the low-cost detection and analysis method of the biomedicine and clinic application fields.A newly developing application of the micro-flow cytometer is the fast detection of the cells or particles with the low concentration.The micro-flow cytometer was self-designed and built based on a micro-fabricated chip that integrated on-chip beam-shaping and microfluidic channels.The experiment of counting micro beads in real time was carried out with the microflow cytometer.And the experiment results show that the linearity correlation coefficients of the micro beads flux against the volume flow and concentration of the sample are over 0.99.The high linearity results prove the high accuracy counting function of the micro-flow cytometer,thus making it a promising candidate for low concentration applications,such as the detection of pathogens in food or water.

关 键 词：微型流式细胞仪 微流体 光学-微流体集成器件 一维水动力学聚焦 微珠计数 

分 类 号：O35[理学—流体力学] TH703[理学—力学]