超临界CO_2流体萃取木耳菌糠最佳工艺的研究  

Study of Optimum Conditions on Supercritical CO_2 Fluild Extraction of Spent Auricularia Auricula Substrate

作　　者：王宗勇 赵轶 何苗 杨慧茹 李萍 薛晓丽 WANG Zongyong;ZHAO Yi;HE Miao;YANG Huiru;LI Ping;XUE Xiaoli(Arts and Sciences of Jilin Agriculture Science and Technology University,Jilin City 132101 China;KeyLabratory of Changbai MountainAnimal and Plant Rescources'Utilization and Protection of Universities in Jilin Province,Jilin City 132101 China)

机构地区：[1]吉林农业科技学院文理学院,吉林吉林132101 [2]吉林农业科技学院 [3]长白山动植物资源利用与保护吉林省高校重点实验室,吉林吉林132101

出　　处：《吉林化工学院学报》2019年第1期5-10,共6页Journal of Jilin Institute of Chemical Technology

基　　金：吉林省大学生科技创新科研项目([2016]No.030)

摘　　要：目的:探讨木耳菌糠超临界CO_2流体萃取的最佳工艺条件及萃取液的化学组成.方法:以萃取率为指标,在单因素试验基础上进行四因素三水平L9(3~4)正交试验确定最佳萃取工艺条件,利用气质联用仪对萃取液的化学成分进行分析.结果:最佳提取工艺条件为萃取压力30 MPa、萃取温度45℃、物料粒度60目、夹带剂体积400 mL,在此工艺条件下萃取率为3.25%.萃取物的化学成分主要由醇、醛、酮、酯、甾类组成,含量最高的三种化合物分别是亚油酸甲酯(5.56%)、藿烯酮(5.26%)、3,5-胆甾烷二烯-7-酮(4.05%).结论:由木耳菌糠提取分离精细化学品是生物质洁净、高效利用的一种新思路.Objective: The optimum conditions for the extraction of Spend Auricularia auricular substrate by supercritical CO2 fluid were studied,and the chemical composition of the extract was analyzed.Methods: Taking the extraction rate as the indicator,orthogonal test of L9( 34) with four factors and three levels was carried out to determine the best conditions on the basis of single factor experiment.The extraction was analyzed with gas chromatograph/mass spectrometer. Results: The results showed that the optimal extraction conditions were30 MPa,45 ℃,60 mesh,400 mL of the entrainer volume and the extraction rate was 3. 25% under these conditions.The chemical composition of extraction can be classified into alcohols,ethers,aldehydes,ketones,organic acids,esters,terpenoids,steroids.The three most abundant compounds were methyl linoleate( 5.56%),17β( H),21β( H)-hop-22( 29)-EN-3-one( 5. 26%),3,5-cholestanedien-7-one( 4. 05%),respectively.Conclusion: Extraction and separation of fine chemicals from spend auricularia auricular substrate is a new idea of clean and efficient utilization of biomass.

关 键 词：木耳菌糠 超临界萃取 工艺优化 化学组分 

分 类 号：TQ911[化学工程]