笋用竹组培快繁及其种苗产业化  

Tissue Culture and Rapid Propagation of Shoot-producing Bamboo and Seeding Industriulization

作　　者：陈国华[1] 陈冬怡 王强 胡群英[1] 黄春华[1] 黄芳[1] 叶秀粦[1] CHEN Guohua;CHEN Dongyi;WANG Qiang;HU Qunying;HUANG Chunhua;HUANG Fang;YE Xiulin(Guangdong Provincial Key Laboratory of Applied Botany,South China Botanical Garden, Chinese Academy of Sciences,Guangzhou Guangdong 510650,China)

机构地区：[1]中国科学院华南植物园广东省应用植物学重点实验室,广东广州510650

出　　处：《农业工程》2018年第11期115-119,共5页AGRICULTURAL ENGINEERING

摘　　要：以苏麻竹等笋用竹的顶芽、侧芽和根等为外植体,通过诱导培养形成脱毒不定芽,通过组培快繁技术,培养出完整的无毒植株。试验结果表明:外植体灭菌处理成功率达78%,诱导不定芽的最适培养基为MS+6-BA3. 0+IBA0. 2+NAA0. 1,不定芽继代增殖的最适培养基为MS+6-BA2. 0+IBA0. 2+NAA0. 1,增殖系数为2. 32,生根率达91%,移栽成活率达93%,为笋用竹种苗提供了一种新型快速的繁殖方式。Terminal buds,lateral buds and roots of some kinds of shoot-producing bamboo like Dendrocalamus latiflorus Munro were taken as explants,adventitious buds were successfully induced by tissue culture,after rapid propagating,complete and virus-free plants were obtained. Results showed that explants sterilization success rate was as high as 78%. Optimal medium for adventitious buds induction was MS + 6-BA3. 0 + IBA0. 2 + NAA0. 1,optimizing medium for subculture multiplication was MS + 6-BA2. 0 + IBA0. 2 + NAA0. 1,and subculture multiplication factor was up to 2. 32,rooting rate was 91% and transplanting survival rate was 93%,is could provide an effect way for rapid propagation of virus-free shoot-producing bamboo seedlings.

关 键 词：笋用竹 快繁 种苗 

分 类 号：S795[农业科学—林木遗传育种]