MITF基因表达与绵羊季节性发情及产羔数关系初探  

Relationship between MITF gene expression and the seasonal estrus and litter size of sheep(Ovis aries)

作　　者：李春艳 狄冉[1] 张子军[2] 任春环[2] 张彦[2] 汤继顺 刘秋月[1] 王翔宇[1] 胡文萍[1] 张效生[3] 张金龙[3] 储明星[1] LI Chunyan;DI Ran;ZHANG Zijun;REN Chunhuan;ZHANG Yan;TANG Jishun;LIU Qiuyue;WANG Xiangyu;HU Wenping;ZHANG Xiaosheng;ZHANG Jinlong;OHU Mingxing(Key Laboratory of Ammal Genetics and Breeding and Reproduction of Ministry of Agriculture/Instttute of Animal ScLence,Chmese Academy of Agricultural Sciences,Beijing 100193,China;College of Animal Sctence and Technology,Anhul Agrtcultural University,HefeL 230036,China;Tianjin Instttute of Animal Sciences,Tianjin 300381,China)

机构地区：[1]中国农业科学院北京畜牧兽医研究所/农业部动物遗传育种与繁殖重点实验室,北京100193 [2]安徽农业大学动物科技学院,合肥230036 [3]天津市畜牧兽医研究所,天津300381

出　　处：《中国农业大学学报》2018年第12期91-96,共6页Journal of China Agricultural University

基　　金：国家自然科学基金项目(31572371);国家肉羊产业技术体系专项(CARS-38);中国农业科学院科技创新工程(ASTIP-IAS13).

摘　　要：为初步探究MITF基因表达和绵羊季节性发情及产羔数的关系,本试验以季节性发情的苏尼特羊(Sunite sheep,SNT)和常年发情的小尾寒羊(Small Tail Han sheep,STH)为研究对象,利用实时荧光定量PCR(qPCR)技术分析SNT在不同光照条件下(短光照、长光照)及STH不同繁殖时期(卵泡期、黄体期)的下丘脑等组织中MITF基因的表达差异及该基因在STH单、多羔母羊卵泡期繁殖器官(卵巢、子宫体、输卵管)中的表达差异。结果表明:1)MITF基因在2个绵羊品种的不同组织中广泛表达且繁殖器官(卵巢、子宫体和输卵管)中该基因表达量较高;2)在长光照条件下SNT垂体中MITF表达量极显著高于短光照条件下表达量(P<0.01);3)STH卵巢中MITF表达量在卵泡期极显著高于黄体期(P<0.01),而子宫体、输卵管中该基因表达量在黄体期显著(P<0.05)或极显著(P<0.01)高于卵泡期;4)单羔组STH子宫体、输卵管中MITF表达水平极显著(P<0.01)或显著(P<0.05)高于多羔组。综上,MITF基因表达与绵羊季节性发情及产羔数存在一定关联,需要进一步进行生物学功能验证。Aiming to tentatively explore the relationship between MITF gene expression and seasonal estrus and litter size in sheep,the real-time fluorescent quantitative PCR(qPCR)method was used to detect the expression difference of MITF gene in nine tissues including hypothalamus in seasonal estrous sheep(Sunite sheep,SNT)at different photoperiods(short photoperiod,long photoperiod)and year-round estrous sheep(Small Tail Han sheep,STH)at different reproduction stages(follicular stage and luteal stage),and the expression difference of MITF gene between single birth tissues and multiple birth tissues including ovary,uterine body and oviduct of STH at follicular stage.Theresults showed that:The MITF gene was widely expressed in different tissues and its’ expression level in ovary,uterine body and oviduct tissues were higher than that in other tissues of two breeds.The expression level of MITF gene in pituitary gland was extremely higher at long photoperiod than that at short photoperiod in SNT(P<0.01).The expression level of MITFin ovary was extremely higher at follicular stage than that at luteal stage(P<0.01),while its expression level in uterine body was significantly higher at luteal stage than that at follicular stage(P<0.05)and its expression in oviduct was extremely higher at luteal stage than that at follicular stage(P<0.01)in STH.The expression level of MITF gene in uterine body in single birth group was extremely higher than that in multiple birth group(P<0.01),while its’ expression level in oviduct in single birth group was significantly higher than that in multiple birth group(P<0.05)of STH at follicular stage.In conclusion,the research results indicated that MITF gene expression may be related to sheep seasonal estrus and litter size and further biological function verification will be required.

关 键 词：绵羊 MITF基因 发情 繁殖时期 组织表达 产羔数 

分 类 号：S826.2[农业科学—畜牧学]