人参皂苷Rg1促进C2C12细胞增殖与分化  

Ginsenoside Rg1 promotes proliferation and differentiation of C2C12 cells

作　　者：徐国超 高岩 马爽[2] 贾胜男[2] 邱晴 王钰 霍洪亮[3] XU Guo-chao;GAO Yan;MA Shuang;JIA Sheng-nan;QIU Qing;WANG Yu;HUO Hong-liang(Basic Education Department,Changchun Vocational Institute of Technology,Changchun 130033,China;School of Life Sciences,Northeast Normal University,Changchun 130024,China;School of Environment,Northeast Normal University,Changchun 130117,China)

机构地区：[1]长春市职业技术学院基础教育学部,吉林长春130033 [2]东北师范大学生命科学学院,吉林长春130024 [3]东北师范大学环境学院,吉林长春130117

出　　处：《东北师大学报（自然科学版）》2018年第4期121-125,共5页Journal of Northeast Normal University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(57478096)

摘　　要：采用MTT比色法、流式细胞术和蛋白质印迹法等实验方法,用不同浓度人参皂苷Rg1孵育小鼠骨骼肌成肌细胞C2C12,研究了人参皂苷Rg1对小鼠骨骼肌成肌细胞C2C12增殖和分化的影响.结果表明:人参皂苷Rg1浓度为0.01mmol/L时能有效促进C2C12细胞生长,提高细胞活力,促进C2C12细胞增殖;Rg1呈浓度依赖性上调肌细胞生成素MyoG表达,增加肌球蛋白重链MyHC合成,促进C2C12细胞分化.This study calculated the influence of the ginsenoside Rg1 for proliferation and differentiation of mouse skeletal myoblasts C2 C12 by using MTT,FCM(flow cytometry)and Western blot.The results indicatedthat Rg1 could effectively improve the cell activity and proliferation of the C2 C12.Furthermore,Rg1 could markedly increase the expression of the myogenin MyoG and myosin heavy chain MyHC,promoted the differentiation of C2 C12 cells.

关 键 词：人参皂苷RG1 增殖 肌球蛋白重链 肌细胞生成素 

分 类 号：Q445[生物学—生理学]