蜜紫菀饮片标准汤剂制备及质量评价方法研究  

Preparation and Quality Evaluation of the Standard Decoction of Asteris Radix et Rhizoma Praeparata Cum Melle

作　　者：张开雪[1] 马伟[1] 熊超[2] 孙伟[2] 李西文[2] 邬兰[2] ZHANG Kai-xue;MA Wei;XIONG Chao;SUN Wei;LI Xi-wen;WU Lan(College of Pharmaceutical Sciences,Heilongjiang University of Chinese Medicine,Harbin 150040,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]黑龙江中医药大学药学院,黑龙江哈尔滨150040 [2]中国中医科学院中药研究所,北京100700

出　　处：《中药材》2018年第4期904-908,共5页Journal of Chinese Medicinal Materials

基　　金：港澳台科技合作专项(2015DFM30030)

摘　　要：目的:建立蜜紫菀饮片标准汤剂质量评价标准,为以蜜紫菀为原料的相关制剂质量标准制定提供参考。方法:收集14批蜜紫菀饮片,制备标准汤剂,测定蜜紫菀标准汤剂出膏率和pH值,并建立HPLC指纹图谱分析方法。结果:14批蜜紫菀标准汤剂出膏率范围为45.8%～61.4%;pH值范围为4.50～4.81;采用"中药色谱指纹图谱相似度评价系统(2012版)"进行指纹图谱分析,相似度均大于0.899,确定12个共有峰,指认其中3个并对其进行简易定量分析,探讨适合标准汤剂定量的指标成分。结论:蜜紫菀标准汤剂研究可为以蜜紫菀为原料的相关制剂质量控制提供参考,因标准汤剂中紫菀酮转移率极低,可选择槲皮素等作为其定量指标成分。Objective:To establish the quality evaluation system for the standard decoction of Asteris Radix et Rhizoma Praeparata Cum Melle,and to provide the reference for quality standard of all the preparations from Asteris Radix et Rhizoma Praeparata Cum Melle.Methods:Fourteen batches of Asteris Radix et Rhizoma Praeparata Cum Melle tablets were collected,the standard decoction was prepared.The extraction rate and pH value were determined and HPLC fingerprint analysis method was established.Results:The extraction rate ranged from 45.8% to 61.4%,and the pH value was at the range of 4.50~4.81.The Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 version)was used to analysis,the similarity was more than 0.899,12 total peaks were determined,of which three were identified.A simple quantitative analysis method was used to discusse the index components for standard decoction.Conclusion:The study of standard decoction of Asteris Radix et Rhizoma Praeparata Cum Melle can provide reference for the related preparation of quality control in Asteris Radix et Rhizoma Praeparata Cum Melle as raw materials,because of shionone has lowly transfer rate for the standard decoction,quercetin can be used as a quantitative index component.

关 键 词：蜜紫菀 标准汤剂 指纹图谱 出膏率 

分 类 号：R284.3[医药卫生—中药学]