HPLC法测定白树植物中α-高野尻霉素的含量  

HPLC Determination of α-homonojirimycin in Suregada Glomerulata(Blume) Baill

作　　者：徐冰 李琼娅 韩晓强 陈若芸[2] 王洪庆[2] Xu Bing;Li Qiongya;HanXiaoqiang;Chen Ruoyun;Wang Hongqing(China Resources Sanjiu,Medical & Pharmaceutical Co.,Ltd.,Shenzhen 518110,China;Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050,China)

机构地区：[1]华润三九医药股份有限公司,广东深圳518110 [2]中国医学科学院北京协和医学院药物研究所,北京100050

出　　处：《生命科学仪器》2018年第6期58-62,29,36,共7页Life Science Instruments

基　　金：深圳市科技计划"重2014-159"技术攻关专项(NO.JSGG20141118144147921)

摘　　要：目的:建立白树植物HPLC含量测定方法。方法:采用反相高效液相色谱法,测定白树中活性成分α-高野尻霉素含量。色谱条件:采用Agilent Extend C18(250×4.6mm,5μm)色谱柱,以甲醇为流动相A,以乙腈为流动相B,水为流动相C,线性梯度洗脱,流速1.2ml/min,柱温25℃,检测波长230nm。结果:α-高野尻霉素与其杂质峰分离度良好,α-高野尻霉素对照品浓度在0.38-18.76(μg/ml)之间线性良好(R=0.9999),平均加样回收率94.9%,RSD值为2.8%(n=6),供试品室温条件下100小时内稳定,检测10批白树植物中α-高野尻霉素含量范围为0.17-0.55%。结论:方法灵敏度高,结果准确,稳定,可作为白树植物的质量控制。Objective:To establish an HPLC method for determination of α-homonojirimycin in Suregada glomerulata(Blume)Baill. Methods: α-homonojirimycin content were determined by RP-HPLC. The chromatographic condition was as follows.The Agilent Extend C18(250×4.6mm,5μm)was adopted, the mobile phase was methanol(A)-acetonitrile(B)-water(c) with gradient elution at a flow rate of 1.2 ml·min-1,the column temperature was 25℃, and the detection wavelength was 230 nm.Results: α-homonojirimycin and the impurities were separated well. The calibration curves of α-homonojirimycin ware linear in the concentration rang(r=1).The average recoveries ofα-homonojirimycin was 94.9%, and RSD(n=6) were 2.8%. The solution was stable for 100 h at room temperature. The content determination results for the 10 batchers of samples were 0.17-0.55%.Conclusion: The method is sensitive,accurate and suitable for quality control ofα-homonojirimycin in Suregada glomerulata(Blume) Baill.

关 键 词：白树 α-高野尻霉素 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]