普通狗牙根与弯穗狗牙根特异分子标记的筛选  

Screening of Specific Molecular Markers for Identification of Cyondon dactylon and C. radiatus

作　　者：黄春琼[1] 刘国道[1] 白昌军[1] 唐军[1] 王文强[1] 丁西朋 Huang Chunqiong;Liu Guodao;Bai Changjun;Tang Jun;Wang Wenqiang;Ding Xipeng(Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China,Ministry of Agriculture,Tropical Crops Genetic Resources Institute,CATAS,Danzhou,571737)

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,农业部华南作物基因资源与种质创制重点实验室,儋州571737

出　　处：《分子植物育种》2018年第24期8121-8127,共7页Molecular Plant Breeding

基　　金：中央级公益性科研院所基本科研业务费专项资金(1630032014028);中国南方草地牧草资源调查(2017FY100601)项目;现代农业产业技术体系建设专项(CARS-35)共同资助.

摘　　要：本研究利用SRAP分子标记结合BAS技术对18份普通狗牙根和28份弯穗狗牙根进行鉴定分析,旨在筛选普通狗牙根和弯穗狗牙根特异分子标记。研究结果显示:从100对SRAP引物中筛选出34对能在普通狗牙根基因池和弯穗狗牙根基因池中扩增出特异条带的引物,其中22对能在普通狗牙根和弯穗狗牙根群体中能稳定扩增出特异标记,共扩增出29个特异标记,普通狗牙根特异标记有4个,弯穗狗牙根特异标记有25个,可将普通狗牙根和弯穗狗牙根区分开。本研究为普通狗牙根与弯穗狗牙根的快速鉴定提供了分子证据。Sequence-related amplifie d polymorph ism(SRAP) molecular marker was used to identify 18 C.dactylon(Cynodon dactylon(Linnaeus) Persoon) and 28 C. radiatus(Cynodon radiatus Roth ex Roemer et Schultes)in the present study. The objective of this study was to screen specific molecular markers between C. dactylon and C. radiatus. The results showed that 34 primer pairs from the original 100 primer pairs were selected based on the specific molecular markers and reproducible bands that they produced between C. dactylon and C. radiatus gene pools, of them, 22 primer pairs could produce specific molecular markers between C. dactylon and C. radiatus groups, and a total of 29 specific molecular markers were amplified. 4 markers were the specific markers of C.dactylon, and 25 markers were the specific makers of C. radiatus. Therefore, these 29 specific molecular markers can be used as molecular evidence to indentify the 2 species, which provided the molecular evidence for rapid identification of C. dactylon and C. radiatus.

关 键 词：普通狗牙根 弯穗狗牙根 特异分子标记 筛选 

分 类 号：Q943.2[生物学—植物学]