五产区牡丹根际及根内放线菌研究  

Research on rhizospheric and endophytic actinomycetes in medicinal tree peony(Paeonia suffruticosa) from five producing regions

作　　者：王雪[1,2,4,5] 管玉鑫 丁东玲[1,2,4,5] 位诗棋 刘辉 郑艳 WANG Xue;GUAN Yu-xin;DING Dong-ling;WEI Shi-qi;LIU Hui;ZHENG Yan(College of Life Sciences,Anhui Normal University,Wuhu 241000,China;Institute of Traditional Chinese Medic.ine Resources,Anhui Normal University,Wuhu 241000,China;College of Environmental Science and Engineering,Anhui Normal University,Wuhu 241000,China;Anhui Provincial Engineering Technology Research Center of Traditional Chinese Medicine Daily Supplies,Wuhu 241000,China;Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources,Wuhu 241000,China)

机构地区：[1]安徽师范大学生命科学学院,安徽芜湖241000 [2]安徽师范大学中药资源研究所,安徽芜湖241000 [3]安徽师范大学环境科学与工程学院,安徽芜湖241000 [4]安徽省中药日化产品工程技术研究中心,安徽芜湖241000 [5]重要生物资源保护与利用研究安徽省省级重点实验室,安徽芜湖241000

出　　处：《中国中药杂志》2018年第22期4419-4426,共8页China Journal of Chinese Materia Medica

基　　金：安徽省自然科学基金项目(1708085MC80)

摘　　要：采用高通量测序技术研究了牡丹皮五产区采收期牡丹根际及根内放线菌多样性。测序获得的129 954条优质序列分为1 754个可操作分类单元,其中从根际土壤中获得的1 311个OTU检出放线菌4纲、4目、27科、67属,Ilumatobacter等33属在五产区根际土壤中均有分布,Longispora等3属仅分布于道地产区,Mycobacterium,Nocardioides,Streptomyces为根际放线菌优势属。根中获得的443个OTU检出放线菌3纲、3目、24科,50属,Cryptosporangium等13属在五产区根中均有分布,Planosporangium,Luteococcus仅分布于道地产区,Nocardioides为根内放线菌优势属。Alpha多样性分析得到亳州、南陵、铜陵产区根际放线菌多样性指数(Chaol,Shannon指数)大于菏泽、洛阳。PCo A分析表明铜陵、南陵产区根际放线菌以及铜陵和洛阳产区根内放线菌组成较为接近。Heatmap显示铜陵、南陵、亳州产区根际放线菌系统发育相似,铜陵、洛阳、南陵产区根内放线菌相似。研究表明牡丹根际、根内放线菌种群组成多样,道地产区根际放线菌系统发育相似性较高。In this study,Paeonia suffruticosa roots and rhizospheric soil in five geographic regions which were harvested in October were utilized as experimental materials,then the diversity of endophytic and rhizospheric actinomycetes were investigated by Highthroughput sequencing technique. The 1 754 operational taxonomic units(OTUs) were obtained from 129 954 high quality sequences,1 311 OTUs were detected in rhizospheric actinomycetes and belonged to four classes,four orders,twenty-seven families and ninetyseven genera,thirty-three genera such as Ilumatobacter were found in the five regions rhizospheric soil while three genera such as Longispora were only detected in the Dao-di regions,the dominant genera were Mycobacterium,Nocardioides,Streptomyces. 443 OTUs were obtained in roots and distributed in three classes,three orders,twenty-four families and fifty genera,thirteen genera such as Cryptosporangium were found in the five regions roots while Planosporangium,Luteococcus were only detected in the Dao-di regions,the dominant genera were Nocardioides. Alpha diversity analysis showed that the Shannon and Chao1 index in rhizospheric actinomycetes in Bozhou,Tongling and Nanling region were higher than Heze and Luoyang. Based on principal co-ordinates analysis(PCo A) analysis,the rhizospheric actinomycetes formations were similar in Tongling and Nanling region,at the same in Tongling and Luoyang endophytic actinomycetes. According to heatmap analysis,Bozhou,Tongling and Nanling region rhizospheric actinomycetes showed a close similarity in actinomycetes community structures on phylogenetic analysis,while Tongling,Luoyang and Nanling endophytic actinomycetes showed the same. Our results not only suggested that the rich and diverse actinomycetes resources in P. suffruticosa roots and rhizospheric soil but also revealed rhizospheric actinomycetes in the Dao-di regions had high similarity.

关 键 词：牡丹 放线菌 多样性 高通量测序 道地性 

分 类 号：S567.15[农业科学—中草药栽培]