UPLC-Q-TOF-MS/MS快速分析杏贝止咳颗粒化学成分  

Rapid identification of chemical components in Xingbei Zhike Keli by UPLC-Q-TOF-MS/MS

作　　者：张宁 高霞[2,3] 周宇 耿婷[2,3] 杨彪[2,3] 王雪[2,3] 黄文哲[2,3] 肖伟 ZHANG Ning;GAO Xia;ZHOU Yu;GENG Ting;YANG Biao;WANG Xue;HUANG Wen-zhe;XIAO Wei(College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Kanion Pharmaceutical Co.,Ltd.,Lianyungang 222001,China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process,Lianyungang 222001,China)

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]江苏康缘药业股份有限公司,江苏连云港222001 [3]中药制药过程新技术国家重点实验室,江苏连云港222001

出　　处：《中国中药杂志》2018年第22期4439-4449,共11页China Journal of Chinese Materia Medica

基　　金：国家“重大新药创制”科技重大专项(2013ZX09402203)

摘　　要：应用UPLC-Q-TOF-MS/MS对杏贝止咳颗粒的化学成分进行分析和鉴定。液相条件:Agilent Zorbax SB-C18(4.6 mm×250 mm,5μm)色谱柱,以甲醇和0.08%甲酸水溶液(含0.1%甲酸铵)为流动相进行梯度洗脱,流速1mL·min^-1,柱温30℃;质谱条件:采用电喷雾(ESI)离子源,正负离子模式下采集数据,根据精确质荷比、二级质谱裂解碎片等信息结合对照品的比对和文献数据进行化合物解析,结果共鉴定和推测出87个化合物,包括生物碱类、黄酮类、香豆素类及皂苷类等,其中23个化合物经对照品验证。该结果为阐明杏贝止咳颗粒的药效物质基础奠定了基础,并为质量控制提供参考。To analyze and identify the chemical components in Xingbei Zhike Keli by using ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry(UPLC-Q-TOF-MS/MS).The analysis was performed on Agilent Zorbax SB-C18(4.6mm×250 mm,5μm)column,with methanol-0.08%formic acid solution(including 0.1%ammonium formate)as the mobile phase for gradient elution.The flow rate was 1mL·min-1and column temperature was 30℃.The MS spectrum was acquired in both negative and positive ion modes by using electron spray ionization(ESI).These components were further analyzed based on accurate m/z,secondary fragmentation and other information combined with reference substance and literature data.As a result,87 compounds were successfully identified and predicted,including alkaloids,flavonoids,coumarins and saponins,of which 23 compounds were verified by comparing with reference substances.These results provide reference for the quality control of Xingbei Zhike Keli,and lay the foundation for elucidating their effective components and mechanism of action.

关 键 词：杏贝止咳颗粒 UPLC-Q-TOF-MS/MS 化学成分谱 

分 类 号：R286.0[医药卫生—中药学]