柴胡饮片中重金属生物可给性研究及其风险评估  

Estimation of bioaccessibility and risk assessment of heavy metals in decoction pieces of Bupleuri Radix

作　　者：吴建杰 骆骄阳[2] 谷善勇 刘好 盛萍[1] 杨美华[2] WU Jian-jie;LUO Jiao-yang;GU Shan-yong;LIU Hao;SHENG Ping;YANG Mei-hua(School of Traditional Chinese Medicine,Xinjiang Medical University,Urumqi 830011,China;Institute of Medicinal Plant Development,Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100193,China)

机构地区：[1]新疆医科大学中医学院,新疆乌鲁木齐830011 [2]中国医学科学院北京协和医学院药用植物研究所,北京100193

出　　处：《中国中药杂志》2018年第22期4479-4485,共7页China Journal of Chinese Materia Medica

基　　金：国家中药标准化项目(ZYBZH-Y-JIN-34);中国医学科学院医学与健康科技创新工程项目(2016-I2M-3-010,2017-I2M-1-013);中医药行业科研专项(201507004-4);国家自然科学基金项目(81560633)

摘　　要：研究不同产地柴胡饮片中重金属整体残留情况以及柴胡饮片摄入而导致的重金属健康风险。基于电感耦合等离子体质谱(ICP-MS)法对山西、陕西、甘肃等产地30批柴胡饮片样品进行重金属含量测定,采用体外消化模型,测定重金属在模拟人体胃肠液生物可给性浓度,结合每日平均摄入量(ADD)和危害商值(HQ)对柴胡饮片的重金属风险进行评估。结果表明,30批柴胡饮片样品中重金属铜(Cu)、铅(Pb)、砷(As)、镉(Cd)、汞(Hg)含量的均未超标,铬(Cr)检出量高,超出美国NSF草案中规定膳食补充剂原料和加拿大规定草药材限量规定。体外消化模型环境中,重金属生物可给性浓度的均值由高到低依次为Cu(5.27 mg·kg^-1)>Cr(4.67 mg·kg^-1)>As(0.18 mg·kg^-1)>Pb(0.12 mg·kg^-1)>Cd(0.06 mg·kg^-1),其中Hg在体外消化模型中未检出。风险评估模型计算成人和儿童最高的总体危害商值(HI)分别为0.799和0.714,成人和儿童的HI均小于1(HI<1)提示本研究中柴胡饮片重金属含量处于安全范围,不会对人体产生明显危害。该文柴胡饮片重金属生物可给性研究相比较重金属总量测定能更准确的判断柴胡饮片中重金属的风险,对我国中药材中重金属的健康风险评估研究具有参考价值。This project was launched to study on the overall residual status of heavy metals of comprehensive understanding in decoction pieces of Bupleuri Radix(DPBR)from different habitats and risk assessment.In this study,inductively coupled plasma mass spectrometry was used to determine the heavy metals of 30 batches of Bupleuri Radix in different producing areas.Simulated gastrointestinal fluid method was used to determine the dissolution rate of heavy metals in the simulated gastrointestinal fluid and the average daily intake Average Daily Dose(ADD)and Hazard Quotient(HQ)index were used to assess the risk of heavy metals in DPBR.The results showed that the contents of copper(Cu),lead(Pb),arsenic(As),cadmium(Cd),and mercury(Hg)in the 30 batches of DPBR didn’t exceed the limit of Chinese Pharmacopeia,however,the chromium(Cr)in DPBR exeeded the limit set by NSF in USA and the limit for herbal ingredients in Canada.The mean bioaccessible heavy metal concentrations decreased from Cu(5.27this test.In addition,cumulative non-carcinogenic health risks(HI)for adults and children were 0.799 and 0.714,respectively.Both HI values in adults and children for combined trace element and heavy metal element exposures were below the value of 1(HI<1),indicating very low carcinogenic health risk.Heavy metals toxicity in herbal medicines and its health risk to humans would be over estimated when assessed only by the total concentrations without considering the bioaccessibility.Therefore,bioaccessibility has grea significance for evaluating the human health risks induced by heavy metals.

关 键 词：柴胡饮片 重金属 体外消化模型 健康风险评估 

分 类 号：R286.0[医药卫生—中药学]