利用RRLC-QqQ-MS^n方法分析比较青皮、陈皮提取物在大鼠尿液及粪便中代谢及排泄过程  

Metabolic and excretory process of Citri Reticulatae Pericarpium Viride and Citri Reticulatae Pericarpium in rats’ urine and feces analyzed by RRLC-QqQ-MS^n

作　　者：刘林林 李辉[1] 何丹[1] 王梦蕾[1] 刘振丽[2] 舒一崧 赵思宇[1] 刘元艳[1] LIU Lin-lin;LI Hui;HE Dan;WANG Meng-lei;LIU Zhen-li;SHU Yi-song;ZHAO Si-yu;LIU Yuan-yan(School of Chinese Materia Medica ,Beijing University of Chinese Medicine,Beijing 100029,China;Institution of Basic Theory,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]北京中医药大学中药学院,北京100029 [2]中国中医科学院中医基础理论研究所,北京100700

出　　处：《中国中药杂志》2018年第22期4519-4527,共9页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81573569)

摘　　要：考察青皮、陈皮醇提物经灌胃给药后,黄酮及香豆素等特征性多酚类成分在大鼠尿液及粪便中的代谢及排泄过程。分别对大鼠灌胃给予青皮及陈皮70%乙醇提取物,收集尿液和粪便。利用RRLC-QqQ-MS^n技术,比较多酚类成分在醇提物、大鼠尿液及粪便中的含量。通过与对照品比较,从青皮、陈皮醇提物中共鉴定了30个多酚类成分,包括黄酮及其苷类、二氢黄酮及其苷类、黄酮醇及其苷类、多甲氧基黄酮类、柠檬苦素类及香豆素类等。各种不同类型化合物,入血后经体循环代谢及肾脏排泄在尿中的检测,较经肠道代谢及排泄在粪便中的检测存在差异。分析结果表明,多甲氧基黄酮类和二氢黄酮苷元类化合物主要经过尿液排出体外,二氢黄酮苷类和柠檬苦素类化合物主要经过粪便排出体外,香豆素类化合物可能经体内代谢转化,在粪便和尿液中几乎均未检测到。This present study was to investigate the metabolism and excretion of characteristic polyphenols such as flavonoids and coumarins in urine and feces of rats after intragastric administration of ethanol extracts of Citri Reticulatae Pericarpium Viride and Citri Reticulatae Pericarpium. The urine and feces of rats were collected after intragastric administration of 70% ethanol extracts of Citri Reticulatae Pericarpium Viride and Citri Reticulatae Pericarpium. Rapid resolution liquid chromatography coupled with quadrupole tandem mass spectrometry(RRLC-QqQ-MS^n) was applied to compare the contents of polyphenols in ethanol extract,urine and feces. By comparing with reference substance,30 polyphenols were identified from the ethanol extracts of Citri Reticulatae Pericarpium Viride and Citri Reticulatae Pericarpium,including flavone glycosides,flavones,flavonone glycosides,flavonones,flavonol glycosides,polymethoxyflavones,coumarins,and limonoids and so on. The detection of various types of compounds showed differences in contents between the intestinal metabolism and excretion in the feces after systemic circulatory metabolism and renal excretion. The results showed that the polymethoxyflavones and flavonones were primarily excreted through urine,and the flavonone glycosides and limonoids were primarily excreted through feces. However,coumarins were hardly detected in feces and urine,indicating that coumarins may be metabolized in the body.

关 键 词：RRLC-QqQ-MS^n 青皮 陈皮 代谢 排泄 

分 类 号：R285.5[医药卫生—中药学]