维吾尔族药大戟脂中三萜类化学成分研究  

Triterpenoids from Uygur medicine latex of Euphorbia resinifera

作　　者：王书云 李国玉[2] 张珂 王航宇 梁宏刚 黄超 黄健 王金辉[2,3,4] 杨宝峰[3] WANG Shu-yun;LI Guo-yu;ZHANG Ke;WANG Hang-yu;LIANG Hong-gang;HUANG Chao;HUANG Jian;WANG Jin-hui;YANG Bao-feng(School of Pharmacy,Henan University,Kaifeng 475004,China;School of Pharmacy, Shihezi University (Key Laboratory of Xinjiang Phytomedicine Resource and Utilization,Ministry of Education), Shihezi 832001,China;Department of Medicinal Chemistry and Natural Medicine Chemistry (State-Prownce Key Laboratories of Biomedicine-Pharmaceutics of China),Harbin Medical University,Harbin 150081,China;School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110015,China)

机构地区：[1]河南大学药学院,河南开封475004 [2]石河子大学药学院新疆植物药资源利用教育部重点实验室,新疆石河子832001 [3]哈尔滨医科大学药化教研室省部共建生物医药工程重点实验室,黑龙江哈尔滨150081 [4]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《中国中药杂志》2018年第18期3688-3693,共6页China Journal of Chinese Materia Medica

基　　金：国家科技支撑计划重点项目(2012BAI30B02);国家“重大新药创制”科技重大专项(2017ZX09305005)

摘　　要：该研究通过反复硅胶柱色谱、ODS柱色谱和半制备型HPLC等方法,从维吾尔族药大戟脂的甲醇超声提取物中共分离得到10个三萜类化合物。利用各种波谱学手段及理化性质确定了这些化合物的结构,分别鉴定为3β-hydroxy-25,26,27-trinoreupha-8-ene-24-oate(1)、iso-maticadienediol(2)、25,26,27-trinortirucall-8-ene-3β-ol-24-acid(3)、dammarendiolⅡ(4)、eupha-8,24-diene-3-ol-26-al(5)、lnonotusane C(6)、eupha-8,24-diene-3β-ol-7,11-dione(7)、inoterpene A(8)、inoterpene B(9)和eupha-24-methylene-8-ene-3β-ol-7,11-dione(10),其中化合物1为新天然产物,化合物2~4首次从该科植物中分离得到,化合物5和6首次从该属植物中分离得到。细胞毒活性测试结果显示化合物1~10对MCF-7,U937以及C6细胞株均未表现出明显的细胞毒活性。Ten triterpenes compounds were isolated from the methanol extraction of the latex of Euphorbia resinifera by means of various chromatographic methods such as silica gel,ODS and semi-preparative HPLC,Their structures were identified by spectroscopic methods and physicochemical properties. These isolated compounds were identified as 3β-hydroxy-25,26,27-trinor eupha-8-ene-24-oate( 1),iso-maticadienediol( 2),25,26,27-trinor Tirucall-8-ene-3β-ol-4-acid( 3),dammarendiol Ⅱ( 4),eupha-8,24-diene-3-ol-26-al( 5),lnonotusane C( 6),eupha-8,24-diene-3β-ol-7,11-dione( 7),inoterpene A( 8),inoterpene B( 9),and eupha-24-methylene-8-ene-3β-ol-7,11-dione( 10). Among them,compound 1 was a new natural product,compounds 2-4 were firstly isolated from the Euphorbiaceae and compounds 5 and 6 were isolated from the genus Euphorbia for the first time. The cytotoxicity of the compounds 1-10 against MCF-7,U937 and C6 cancer cell lines was evaluated,but none of the compounds was active.

关 键 词：维吾尔族药 大戟脂 化学成分 三萜 细胞毒活性 

分 类 号：R29[医药卫生—民族医学]