基于UPLC-Q-TOF-MS的五味子保肝作用代谢组学研究  

Metabonomics study on hepatoprotective effect of Schisandrae Chinensis Fructus based on UPLC-Q-TOF-MS

作　　者：周永峰[1] 牛明[1] 房吉祥[1] 马艳芹[1] 崔园园 董旖[1] 张蓉蓉[1] 王伽伯[1] 张萍[1] ZHOU Yong-feng;NIU Ming;FANG Ji-xiang;MA Yan-qin;CUI Yuan-yuan;DONG Yi;ZHANG Rong-rong;WANG Jia-bo;ZHANG Ping(302 Military Hospital of China,Beijing 100039,China)

机构地区：[1]解放军第302医院

出　　处：《中国中药杂志》2018年第18期3756-3763,共8页China Journal of Chinese Materia Medica

基　　金：全军后勤科研计划面上项目(CWS14J072);军民标准通用化工程项目(BWS17B038)

摘　　要：该文旨在考察五味子对CCl4致小鼠肝损伤的保护作用,探讨其对血清中代谢产物的影响,寻找五味子保肝功效的潜在生物标志物,以期初步探索五味子保肝作用的科学内涵。采用CCl4建立小鼠肝损伤模型,观察小鼠肝组织病理切片,测定小鼠血清中ALT,AST等生化指标的含量,采用基于UPLC-Q-TOF-MS的代谢组学技术,并结合主成分分析(PCA)和正交校正的偏最小二乘判别分析(OPLS-DA)等方法筛选、鉴定肝损伤相关的生物标志物。结果表明空白组、模型组以及给药组的代谢物能够显著区分,发现并初步鉴定了50个差异化合物并富集了7条五味子保肝功效可能相关的代谢通路。该实验进一步验证了五味子的保肝功效,并初步探讨了五味子的保肝功效可能与氨基酸代谢,维生素代谢以及甘油磷脂代谢等相关代谢通路有关。To investigate the hepatoprotective effect of Schisandrae Chinensis Fructus( SCF) on CCl4-induced liver injury,observe its effect on serum metabolites,explore its scientific connotation in liver preservation and find the biomarkers for hepatoprotective effect of SCF. Liver injury model was established by using CCl4. The pathological sections of liver tissues were observed and the contents of alanine transaminase( ALT) and aspartate transaminase( AST) in serum were determined. The metabolic skills were adopted based on ultra performance liquid chromatography time-of-flight mass spectrometry( UPLC-Q-TOF-MS),principal component analysis( PCA)and orthogonal partial least squares discriminant analysis( OPLS-DA) for screening and identification of biomarkers related to liver injury. The results showed the metabolites in blank group,model group and administration group could be easily distinguished,50 differential compounds were identified and 7 possible metabolic pathways of liver protection were enriched. In this experiment,the hepatoprotective effect of SCF was verified,and the related metabolic pathways such as amino acid metabolism,vitamin metabolism and glycerophospholipid metabolism were discussed.

关 键 词：五味子 代谢组学 保肝 UPLC—Q—TOF—MS 

分 类 号：R285.5[医药卫生—中药学]