土壤水分含量对羌活植株生长及有效成分积累的影响  

Effects of soil water content on growth and accumulation of active ingredients of Notopterygium incisum

作　　者：杨萍 王红兰[2] 张燕 蒋舜媛[1,2] 杜玖珍 孙洪兵[2] 周毅[2] 孙辉[3] YANG Ping;WANG Hong-lan;ZHANG Yah;JIANG Shun-yuan;DU Jiu-zhen;SUN Hong-bing;ZHOU Yi;SUN Hui(College of Pharmacy,Southwest Medical University,Luzhou 646000,China;Sichuan Academy of Chinese Medicine Sciences, Chengdu 610041,China;Department of Environmental Science and Engineering,Sichuan University,Chengdu 610055,China;Department of Pharmacy,the First People's Hospital of Zigong,Zigong 543000,China)

机构地区：[1]西南医科大学药学院,四川泸州646000 [2]四川省中医药科学院,四川成都610041 [3]四川大学环境科学与工程系,四川成都610065 [4]自贡市第一人民医院,四川自贡643000

出　　处：《中国中药杂志》2018年第24期4824-4830,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81473311);国家重点研发计划项目(2017YFC1700705)

摘　　要：为探讨水分对羌活植株生长的影响,以一年生羌活植株为供试材料,通过盆栽实验,测定不同土壤水分下羌活植株生长、生理及有效成分变化特征指标。结果表明:(1)含水量保持在100%FC水平有利于羌活植株生长与生物量积累。100%FC下羌活植株叶长、叶柄长、叶柄粗、根长、根粗和地下干生物量均显著增加,70%FC对植株产生轻度胁迫,20%FC不能提供植株生长所需的水分,植株全部干枯死亡。(2)随着水分处理时间的延长,羌活植株叶片叶绿素相对含量(SPAD)、根茎过氧化物酶(POD)活性均先升高后降低;根茎超氧化物歧化酶(SOD)活性呈上升的趋势;根茎丙二醛(MDA)含量则先减后增。实验后期,40%FC下羌活植株SPAD,POD,SOD活性低于70%FC和100%FC,MDA含量则高于70%FC和100%FC。(3)含水量在70%FC有利于羌活植株有效成分积累。70%FC下羌活植株羌活醇、异欧前胡素含量显著高于40%,100%FC。综上所述,土壤水分含量为70%~100%FC时,有利于羌活植株生长和有效成分的积累。To investigate the adaptive mechanism of Notopterygium incisum to water changes,one-year-old seedlings were used as test materials. The characteristics of growth,physiology,and active ingredients of N. incisum were studied at different soil water content by pot experiments. The result showed that:(1)The water content at 100% field capacity is conducive to the growth and accumulation of biomass of N. incisum. The leaf length,petiole length,petiole diameter,root diameter,root length and biomass of underground dry of N. incisum were all significantly increased at the 100% field capacity,and they slightly declined at 70% field capacity. 20%field capacity could not provide the necessary water to growth of N. incisum,all N. incisum withered and died.(2)With the extension of moisture treatment time,the relative content of chlorophyll( SPAD) in leaves and the activity of peroxidase( POD) in rhizome showed a trend of increasing firstly,decreasing then;the tendency of activity of superoxide dismutase( SOD) in rhizome increased;the content of malondialdehyde( MDA) in rhizome decreased firstly and increased then;. For the late test,the SPAD value,the activity of POD and SOD in N. incisum at the 40% field capacity were lower than 70% field capacity and 100 field capacity,while the content of MDA in rhizome were higher than 70% field capacity and 100% field capacity.(3) At 70% field capacity,the active ingredients accumulated most in the N. incisum. At the 70% field capacity,the notopterol and isoimperatorinwerehigher than100 field capacity and 40 field capacity. In summary,the soil water content at 70%-100% field capacity would contribute to the growth and accumulation of active ingredients of N. incisum.

关 键 词：羌活 土壤水分 解剖结构 SPAD值 保护酶 MDA 

分 类 号：S567.239[农业科学—中草药栽培]