一种鉴定西红花和红花源性成分的实时荧光PCR方法  

A real-time fluorescence quantitative PCR method for source identification of Crocus sativus and Carthamus tinctorius

作　　者：张全芳 刘艳艳 谭晴晴 姜欣欣 陈雪燕 葛付存 步迅 张永清 ZHANG Quan-fang;LIU Yan-yah;TAN Qing-qing;JIANG Xin-xin;CHEN Xue-yan;GE Fu-cu.n;BU Xun;ZHANG Yong-qing(Biotechnology Research Center,Shandong Academy of Agricultural Sciences/Laboratory of Applied Life Science,Shandong Academy of Agricultural Sciences,Ji'nan 250100,China;Shandong Baiweitang Chinese Medicine Pieces Co.,Ltd.,Ji'nan250108,China;Instituteof Natural Medicine,Shandong University of Traditional Chinese Medicine,Ji'nan 250355,China)

机构地区：[1]山东农业科学院生物技术研究中心应用生命科学实验室,山东济南250100 [2]山东省百味堂中药饮片有限公司,山东济南250108 [3]山东中医药大学药学院天然药物研究所,山东济南250355

出　　处：《中国中药杂志》2018年第23期4575-4581,共7页China Journal of Chinese Materia Medica

基　　金：国家中医药行业科研专项(201407002);山东省重点研发计划项目(公益性科技攻关类)

摘　　要：该研究根据GenBank中的ITS2序列设计引物,基于SYBRGreen荧光PCR技术建立了一种能快速、灵敏和特异的鉴别西红花和红花源性的实时荧光定量PCR检测体系。与中草药DNA条形码技术相比,该方法检测时间短、特异性强、灵敏度高。利用该方法检测15份样品,其中4份为红花,8份样品为西红花,3份为其他源性,与中草药DNA条形码检测结果一致。该方法为药材中西红花与红花源性的快速鉴定奠定了基础。The specific PCR primer was designed base on ITS2 sequence in GenBank, and we developed a SYBRGreen real-time fluorescence quantitative PCR system for identification of Crocus sativus and Carthamus tinctorius source. Compared with Chinese herbal medicine DNA barcode technique, this method showed characteristics of shorter time, higher specificity and sensitivity. Using this method to detect 15 samples, 4 were C. sativus, 8 were C. tinctorius, and the other 3 samples were none of them. The result was in accordance with Chinese herbal medicine DNA barcode. This study lays the foundation for identification of related Chinese medical materials.

关 键 词：sYBRGreen实时荧光定量PCR 西红花 红花 源性鉴定 

分 类 号：S567.2[农业科学—中草药栽培]