脑心通胶囊肠吸收液抗ADP诱导血小板凝集的有效成分辨识研究  

Identification of effective components from Naoxintong Capsules intestinal absorption liquid in inhibiting ADP-induced platelet aggregation

作　　者：郭宇飞[1] 赵晔[1] 何子龙[1] 方文娟 吴宏伟[1] 张方博[1] 杨洪军[1] GUO Yu-fei;ZHAO Ye;HE Zi-long;FANG Wen-juan;WU Hong-wei;ZHANG Fang-bo;YANG Hong-jun(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院中药研究所

出　　处：《中国中药杂志》2018年第23期4639-4644,共6页China Journal of Chinese Materia Medica

基　　金：国家“重大新药创制”科技重大专项(2011ZX09201-201-26)

摘　　要：应用外翻肠囊法制备肠吸收液,同时拆方或构建重组方,生化试剂盒结合酶标仪检测ADP诱导血小板的聚集率,寻找脑心通胶囊组方中拮抗血小板聚集的单味或多味中药。研究表明脑心通胶囊能抑制ADP诱导血小板聚集。通过拆方以及重组方研究,表明丹参、赤芍、桂枝以及水蛭是脑心通全方抗血小板聚集的主要活性中药。此4味中药又以桂枝在全方中发挥主要的抗血小板聚集作用。桂枝肠吸收液来源的香豆素在50~200μmol·L^-1时拮抗血小板聚集。综上所述,丹参、赤芍、桂枝以及水蛭是脑心通胶囊抗ADP诱导血小板聚集的主要活性成分,以桂枝抗血小板聚集作用最强。Intestinal absorption liquid was prepared by using everted intestinal sac method;meanwhile, its recipes were decomposed or restructured. Platelet aggregation activity was examined by biochemical tests and a microplate reader. One or more kinds of Chinese medicines which displayed inhibiting activity in Naoxintong Capsules were screened through separation and combination of prescription. The results showed that Naoxintong Capsules could inhibit ADP-induced platelet aggregation. Recipe decomposition and restructuring results showed that Salviae Miltiorrhizae Radix et Rhizoma, Paeoniae Radix Rubra, Cinnamomi Ramulus and Hirudo were the main effective medicines in inhibiting platelet aggregation. Furthermore, Cinnamomi Ramulus played a vital role in inhibiting activity among those four kinds of Chinese medicines. Coumarin derived from intestinal absorption liquid of Cinnamomi Ramulus had inhibiting activity in the range of 50-200 μmol·L^-1, and other ingredients such as cinnamyl alcohol and cinnamaldehyde also had inhibiting activities. In conclusion, Salviae Miltiorrhizae Radix et Rhizoma, Paeoniae Radix Rubra, Cinnamomi Ramulus and Hirudo are the main components for inhibiting ADP-induced platelet aggregation, and Cinnamomi Ramulus has the most strongest inhibiting activity in Naoxintong Capsules.

关 键 词：脑心通胶囊 肠吸收液 ADP 血小板聚集 

分 类 号：R285[医药卫生—中药学]