西洋参实时荧光定量PCR快速鉴别方法的建立  

Establishment of a quantitative real-time PCR for rapid detection of Panax quinquefolius

作　　者：黄永辉[1] 徐淼锋[1] 权永兵[1] 迟远丽[1] 林伟 廖力[1] 张卫东[1] HUANG Yong-hui;XU Miao-feng;QUAN Yong-bing;CHI Yuan-li;LIN Wei;LIA0 Li;ZHANG Wei-dong(Technology Center of Zhuhai Entry-Exit Inspection and Quarantine Bureau,Zhuhai 519000,China)

机构地区：[1]珠海出入境检验检疫局技术中心

出　　处：《中国中药杂志》2018年第20期4050-4054,共5页China Journal of Chinese Materia Medica

基　　金：珠海出入境检验检疫局科技计划项目(ZH2016-3,ZH2017-24);珠海进出口公共技术服务平台产学研协同创新计划项目(IETP201701009)

摘　　要：基于ITS2片段序列特征,该研究设计了西洋参的特异性引物、探针,通过条件的优化建立了西洋参的实时荧光定量PCR快速鉴别方法。利用该方法对43个中药材样本进行了验证,结果能准确地鉴别出43个药材样本中的22个西洋参,并且灵敏度高,检测限可达到1×10-4ng。该方法具有准确、快捷、灵敏的特点,可对西洋参及其加工产品进行准确的鉴别和检测,应用前景广阔。In this study,the specific primers and probes of Panax quinquefolius were designed for a quantitative real-time PCR,and the rapid identification method of P. quinquefolius was established by optimizing conditions. The method was used to validate 43 samples of the traditional Chinese medicine,and the results showed that 22 samples of P. quinquefolius were identified accurately. The limit of detection of the method can be reach to 1 × 10-4 ng. The method is accurate,fast,sensitive and specifically.

关 键 词：西洋参 人参 实时荧光定量PCR 快速鉴别 

分 类 号：R284.1[医药卫生—中药学]