胃癌患者血清IL-32、TNF-α表达意义研究  

Expression and clinical significance of serum IL-32 and TNF-αin patients with gastric cancer

作　　者：杜三军[1] 李多[1] 于永强[1] 吴娜[1] DU San-jun;LI Duo;YU Yong-qiang;WU Na(Department of Gastroenterology,the First Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei 075000,China)

机构地区：[1]河北北方学院附属第一医院消化内科,河北张家口075000

出　　处：《中国预防医学杂志》2018年第12期933-936,共4页Chinese Preventive Medicine

基　　金：河北省张家口市科技计划项目(1821049D)

摘　　要：目的探讨胃癌患者白细胞介素-32 (IL-32)和肿瘤坏死因子-α(TNF-α)水平,并分析其与幽门螺杆菌感染及理化因素之间的关系。方法选取2017年6月至2018年8月,河北北方学院附属第一医院胃癌患者90例,采集空腹外周静脉血,检测血清IL-32和TNF-α水平,幽门螺杆菌和细胞毒素相关蛋白(CagA)。比较CagA阳性胃癌患者、CagA阴性胃癌患者和幽门螺杆菌阴性胃癌患者血清IL-32和TNF-α的差异。结果胃癌组患者与对照组患者IL-32 (F=43.365,P<0.01)与TNF-α(F=87.786,P<0.01)水平差异有统计学意义。CagA+胃癌患者、CagA-胃癌患者、HP-胃癌患者IL-32 (F=41.066,P<0.01)与TNF-α(F=27.427,P<0.01)水平差异有统计学意义。低分化胃癌患者血清IL-32、TNF-α水平均高于中高分化患者,差异有统计学意义(t=3.374、4.376,P<0.01)。远处转移患者血清IL-32、TNF-α高于无转移患者,差异有统计学意义(t=5.469、7.727,P<0.01)。结论幽门螺杆菌感染是导致胃癌患者血清IL-32、TNF-α升高的危险因素,CagA在其中发挥着重要作用,检测血清IL-32对胃癌具有良好的诊断作用。Objective To discuss the expression and clinical significance of serum IL-32 and TNF-αin patients with gastric cancer and to understand the the relationship with Helicobacter pylori infection. Methods 90 patients with gastric cancer were enrolled in the First Hospital of Hebei North University from June 2017 to August 2018,blood samples were collected to determine the serum levels of interleukin(IL)-32,tumor necrosis factor(TNF)-αlevels,and cytotoxin-associated protein(CagA).The differences of serum levels of IL-32 and TNF-αwere compared among CagA-positive,CagA-negative and H.pylori-negative patients. Results The differences of serum levels of IL-32 and TNF-αwere statistically significant among CagA-positive gastric cancer patients,CagA-negative patients and H.pylori-negative gastric cancer patients(F =41.066,27.427,P<0.01).The serum levels of IL-32 and TNF-αin patients with poorly differentiated and distant metastasis were higher than those with well-differentiated and no cancer metastasis patients(t=3.374,4.376,5.469,7.727,P<0.01). Conclusions H.pylori infection is a risk factor for IL-32 and TNF-αelevation in patients with gastric cancer,in which CagA plays an important role.Serum IL-32 detection may help for the diagnosis of gastric cancer.

关 键 词：胃癌 幽门螺杆菌 细胞毒素相关蛋白 白细胞介素-32 肿瘤坏死因子-Α 

分 类 号：R735.2[医药卫生—肿瘤]