A WW Domain-Containing Protein Forms Immune Nuclear Bodies against Begomoviruses  

作　　者：lara P. Calil lana P.S. Quadros Thais C. Araujo Christiane E.M. Duarte Bianca C. Gouveia-Mageste Jose Cleydson F. Silva Otavio J.B. Brustolini Ruan M. Teixeira Caue N. Oliveira Rafael W.M.M. Milagres Gilberto S. Martins Joanne Chory Pedro A.B. Reis Joao Paulo B. Machado Elizabeth P.B. Fontes 

机构地区：[1]Departament of Biochemistry and Molecular Biology, Universidade Federal de Vigosa, Vigosa, Minas Gerais 36570-000, Brazil [2]Nati onal In stitute of Science and Tech no logy in Plant-Pest Interactions, Bioagro, Universidade Federal de Vicosa, Vinosa, Minas Gerais 36570-000, Brazil [3]Departament of Genetics, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil [4]Howard Hughes Medical Institute and Plant Biology Laboratory, The Salk Institute of Biological Studies, La Jolla, CA 92037, USA [5]Agronomy Institute, Universidade Federal de Vigosa, Campus Florestal, Florestal, Minas Gerais 35690-000, Brazil [6]These authors contributed equally to this article

出　　处：《Molecular Plant》2018年第12期1449-1465,共17页分子植物（英文版）

摘　　要：The bipartite begomoviruses (Geminiviridae family), which are DNA viruses that replicate in the nucleus of infected cells, encode the nuclear shuttle protein (NSP) to facilitate the translocation of viral DNA from the nucleus to the cytoplasm via nuclear pores. This intracellular trafficking of NSP-DNA complexes is accessorized by the NSP-interacting guanosine triphosphatase (NIG) at the cytosolic side. Here, we report the nuclear redistribution of NIG by AtWWPI, a WW domain-containing protein that forms immune nuclear bodies (NBs) against begomoviruses. We demonstrated that AtWWPI relocates NIG from the cytoplasm to the nucleus where it is confined to AtWWP1-NBs, suggesting that the NIG-AtWWP1 interaction may interfere with the NIG pro-viral function associated with its cytosolic localization. Consistent with this assumpti on joss AtWWPI functi on cuased plants more susceptible to begomovirus infection, whereas overexpression of AWWP1 enhaneed plant resistance to begomovirus. Furthermore, we found that a mutant versi on of AtWWPI defective for NB formatio n was noIonger capable of interacti ng with and relocating NIG to the nucleus and lost its immune function against begomovirus. The antiviral function of AtWWP1-NBs, however, could be antagonized by viral infection that induced either the disruption or a decrease in the number of AtWWP1-NBs. Collectively, these results led us to propose that AtWWPI organizes nuclear structures into nuclear foci, which provide intrinsic immunity against begomovirus infection.

关 键 词：nuclear BODIES ANTIVIRAL immunity BEGOMOVIRUS NIG AtWWP1 

分 类 号：Q[生物学]