潼白蒺藜药对调节高脂血症模型大鼠血脂作用及机制研究  

Study on the Lipid-lowering Effect and Potential Mechanism of the Couplet Medicines of Flastem Milkvetch Seed and Tribulus Terrestris on Hyperlipidemia Rats

作　　者：杨爱玲[1] 樊华[1] 王佑华[1] 彭珑萍 周莉 汤诺[1] 邓兵[1] 周端[1] Yang Ailing;Fan Hua;Wang Youhua;Peng Longping;Zhou Li;Tang Nuo;Deng Bing;Zhou Duan(Department of Cardiology,Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China;Department of Traditional Chinese Medicine, Xuhui Central Hospital of Shanghai,Shanghai 200031,China)

机构地区：[1]上海中医药大学附属龙华医院心内科,上海200032 [2]上海市徐汇区中心医院中医科,上海200031

出　　处：《世界科学技术-中医药现代化》2018年第11期2008-2013,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家科技部科技支撑计划项目(2012BAI41B05):慢性非传染性疾病防控领域中医预防保健(治未病)服务技术应用示范;负责人:周端;上海市科委科研计划项目(15401902500):基于斑马鱼平台潼白蒺藜药对组分配伍治疗高胆固醇血症的候选药物研究;负责人:王佑华

摘　　要：目的:探究潼白蒺藜对高脂血症模型大鼠血脂水平的影响及机制,为药对协同降血脂的临床应用提供参考。方法:48只SPF级SD大鼠随机等分为正常对照组,高脂模型组,辛伐他汀组、潼白蒺藜药对组,并设白蒺藜组,潼蒺藜组对比。正常对照组采用基础饲料喂养,其余各组采用高脂饲料饲喂法建立高脂血症大鼠模型,造模的同时预防性给药,用潼蒺藜水提物、白蒺藜水提物、潼白蒺藜药对水提物和阳性对照药辛伐他汀分别灌胃,6周后检测大鼠体重及各脏器指数、并检测血清总胆固醇(Total cholesterol,TC)、甘油三酯(Triglyceride,TG)、高密度脂蛋白(high density lipoprotein-cholesterol,HDL-C)、低密度脂蛋白(high density lipoprotein-cholesterol,LDL-C)的含量,RT-PCR及western blot检测各组肝脏组织三羟基三甲基戊二酸单酰辅酶A还原酶(3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase,HMGCR)、胆固醇7a羟化酶(Cytochrome P450 7A1,CYP7A1)、低密度脂蛋白受体(Low-Density Lipoprotein Receptor,LDL-R)mRNA及蛋白表达。结果:三组中药组均不同程度降低高脂模型大鼠血液TC、TG、LDL-C含量,提高HDL-C含量,其中潼白蒺藜药对更加显著(P<0.01);三组中药组均能提高脂模型大鼠肝脏HMGCR、CYP7A1、LDL-R基因及蛋白表达,潼白蒺藜药对组疗效更为显著。结论:潼白蒺藜药对能显著调节机体的血脂水平,有效预防高脂血症,发挥协同增效作用,且其机制可能与调节HMGCR、CYP7A1、LDL-R表达有关。Objective: To explore the lipid-lowering effect and potential mechanism of the couplet medicines of Flastem Milkvetch Seed and Tribulus terrestris on hyperlipidemia rats. Methods: Forty-eight Sprague-Dawley rats were randomly divided into 6 groups, normal control group, high-fat model group, simvastatin group, Tribulus terrestris group, Flastem Milkvetch Seed group, and couplet medicines of Flastem Milkvetch Seed and Tribulus terrestris group, with 8 rats in each group. The normal control group was fed with basal diet, and the other groups were fed with high-fat diet to establish a hyperlipidemia rat model. At the same time, different group rats were treated with different drug respectively. 6 weeks later, the body weight and visceral index of the rats were measured. Levels of serum cholesterol(TC), triglyceride(TG),high density lipoprotein cholesterol(HDL-C), and low density lipoprotein cholesterol(LDL-C) were detected by automatic biochemical analyzer. The mRNA and protein expression of HMG coenzyme A reductase(HMGCR), cholesterol 7-alpha hydroxy-lase(CYP7A1) and low density lipoprotein receptor(LDL-R) were detected by RT-PCR and western blot.Results: Compared with model group, the serum levels of TC, TG, and LDL-C were significantly decreased in all Chinese medicine groups, and the serum level of HDL-C was significantly increased in Flastem Milkvetch Seed group and couplet medicines of Flastem Milkvetch Seed and Tribulus terrestris group. Moreover, the mRNA and protein expression of HMGCR, CYP7A1, and LDL-R in rat liver also significantly increased in all Chinese medicine treatment groups. And couplet medicines of Flastem Milkvetch Seed and Tribulus terrestris group shows more significantly effect. Conclusion:Couplet medicines of Flastem Milkvetch Seed and Tribulus terrestris significantly regulate the serum lipid levels on hyperlipidemia rat model, and its mechanism may be related to the regulating expressions of HMGCR, CYP7A1, LDL-R gene.

关 键 词：高脂血症 药对 潼蒺藜 白蒺藜 胆固醇 

分 类 号：R285.2[医药卫生—中药学]