银蓝调脂胶囊对巨噬细胞泡沫化的抑制作用及机制研究  

Study on the Effects and Mechanism of Yinlan Tiaozhi Formula on Macrophage Foaming

作　　者：黄丹娥 李茹月 蔡大可 姚楠 甘海宁 曾晓会 陈玉兴 Huang Dane;Li Ruyue;Cai Dake;Yao Nan;Gan Haining;Zeng Xiaohui;Chen Yuxing(Guangdong Research Institute of Traditional Chinese Medicine Manufacturing Technology,Guangzhou 510095, China;Guangzhou University of Traditional Chinese Medicine,Guangdong Second Traditional Chinese Medicine Hospital,Guangzhou 510275,China)

机构地区：[1]广东省中医药工程技术研究院,广州510095 [2]广州中医药大学广东省第二中医院,广州510275

出　　处：《世界科学技术-中医药现代化》2018年第11期2014-2020,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家科技部重大科技专项(2016ZX09101076):银蓝调脂胶囊作用机理及临床和产业化关键技术研究;负责人:孙冬梅;广东省科技计划项目(2015A040404030):广东省调脂创新中药研发技术服务平台的建设;负责人:陈玉兴;广东省中医药局科研项目(20161026):基于PPARα-LXRα-ABCA1通路研究银蓝调脂胶囊对胆固醇外排的影响及机理;负责人:黄丹娥

摘　　要：目的:基于LXRα-ABCA1通路和炎症机制研究银蓝调脂方对巨噬细胞泡沫化的抑制作用及机制。方法:采用ox-LDL(50 mg·L-1)处理RAW264.7细胞构建巨噬细胞泡沫化模型,制备银蓝调脂胶囊含药血清,细胞分为空白组、模型组、给药组。药物干预后,MTT法检测细胞增殖,利用油红O染色观察细胞内脂质堆积情况,GPO-PAP法测定细胞内总胆固醇含量,实时荧光定量PCR(RT-qPCR)法检测mRNA表达、Westernblotting检测蛋白的表达。结果:与模型组比较,银蓝调脂胶囊能显著抑制巨噬细胞泡沫化、减少脂质堆积,显著上调LXRα、ABCA1基因及蛋白表达,同时抑制炎症因子COX2、iNOS基因及蛋白的表达。结论:银蓝调脂胶囊对巨噬细胞泡沫化的具有抑制作用,可能与抑制炎症反应、上调LXRα-ABCA1通路有关。Objective: To investigate the inhibitory effect of macrophage foaming by Yinlan Tianzhi formula(YLTZ) and to explain its effects on lipid-induced inflammation and LXRα-ABCA1 signal pathway. Methods: The model of macrophage foaming was induced by incubating the RAW264.7 cells or BMMs with ox-LDL(50 mg·L^-1). The serum containing YLTZ was prepared. The cells were divided into blank group, model group, and drug group. After drug intervention, MTT method was used to detect cell proliferation. The lipid accumulation in cells was observed by oil red O staining, and GPO-PAP method was used to determine the total cholesterol content in cells. Protein and mRNA levels were determined by Western blot and RT-qPCR. Results: Compared with control group, after YLTZ treatment, the lipid level was significantly decreased, and the level of mRNA and protein of LXRα and ABCA1 were significant increased. The expression of inflammatory factor COX2 and iNOS was significantly decreased. Conclusion: YLTZ inhibits macrophage foaming through enhancing LXRα-ABCA1 pathway and suppressing of inflammatory response.

关 键 词：银蓝调脂胶囊 泡沫细胞 胆固醇逆向转运 炎症 

分 类 号：R285.6[医药卫生—中药学]