皖贝母产生物碱内生真菌的筛选与鉴定  

Selection and Identification of Producing Alkaloid Endophytic Fungi From Fritillaria anhuiensis

作　　者：许勇[1] 戴陈伟 董朝阳[1] 蔡标[1] Xu Yong;Dai Chenwei;Dong Chaoyang;CaiBiao(Institute of Microbiology,Anhui Academy of Medical Sciences,Hefei 230061,China)

机构地区：[1]安徽省医学科学研究院微生物研究所,合肥230061

出　　处：《世界科学技术-中医药现代化》2018年第11期2021-2026,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：安徽省卫生和计划生育委员会"十三五"医疗卫生重点专科(皖卫科教[2017]30号):病原微生物实验室;负责人:蔡标;安徽省卫生计生委中医药科研课题(2014zy46):皖贝母内生真菌分离与鉴定;负责人:董朝阳

摘　　要：目的:从皖贝母中分离产生物碱的内生真菌。方法:采用组织块法、薄层层析、高效液相色谱-旋转蒸发光检测器法以及形态学和分子生物学方法从皖贝母鳞茎中筛选出产生物碱的内生真菌。结果:从皖贝母中分离出1株产生物碱的内生真菌,并鉴定为极细链格孢菌(Alternaria tenuissima)。结论:从皖贝母中分离到了一株产生物碱内生真菌bm-2,为解决皖贝母药用资源匮乏的问题提供理论依据。Objective: To select alkaloid-producing endophytic fungi strains from Fritillaria anhuiensis. Methods: The endophytic fungi were selected from the bulbus of Fritillaria anhuiensis, by tissue explants, thin layer chromatography,the high performance liquid chromatography and high performance liquid chromatography-evaporative light scattering detection, morphologic observation and molecular biological methods. Results: One strain as an endophytic fungi capable of producing alkaloid was selected from the bulbus of Fritillaria anhuiensis, and was classified as Alternaria tenuissima.Conclusion: The selected alkaloid-producing endophytic fungi strain bm-2 from Fritillaria anhuiensis provide theoretical foundation for solving the problem of the deficient medical resources of Fritillaria anhuiensis.

关 键 词：皖贝母 内生真菌 生物碱 

分 类 号：R281.4[医药卫生—中药学]