基于Shotgun液相质谱联用技术研究膜蛋白组学的进展  

Advances in Liquid Chromatography-mass Spectrometry-based Shotgun Membrane Proteomics

作　　者：孔德志[1] 任雷鸣[1] 张炜[1] Kong Dezhi;Ren Leiming;Zhang Wei(College of Chinese integrative medicine,Hebei medical university,Shijiazhuang 050017,China)

机构地区：[1]河北医科大学中西医结合学院,石家庄050017

出　　处：《世界科学技术-中医药现代化》2018年第8期1329-1336,共8页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委面上基金项目(81573416):TARPs对AMPA受体功能的调节;负责人:张炜;河北省自然科学基金委专项项目(H2018206641):蛋白激酶A调控脂代谢稳态的神经与分子组学机制研究;负责人:张炜

摘　　要：膜蛋白是细胞膜功能的主要承担者,参与信息传递、物质交换、催化反应等生物学功能,也是60%-70%药物的主要作用靶点。正是基于膜蛋白的重要性,以研究整体膜蛋白水平的膜蛋白组学(membraneproteomics)逐渐兴起,并发展成为蛋白质组学研究生物学功能中的重要组成部分。由于膜蛋白的疏水性、低丰度等特点,在进行Shotgun质谱分析前仍需要对样品进行一些特殊的处理,本文针对膜蛋白样品的前处理及相关应用研究的进展进行了综述。Membrane protein(PMs) plays a key role in the biological function of plasma membrane,and takes part in information transmission,material exchange,catalytic reaction,etc.More importantly,many PMs are the targets for about60%-70% approved drugs.Because of the importance of PMs,more and more researchers are focusing on the membrane proteomics which can simultaneously reflect the change of thousands of proteins.As a part of proteomics,however,membrane proteomics have some difficulties in sample preparation prior to LC-MS analysis because the PMs are lowabundance and high-hydrophobicity.In the review,we aimed at the advances of sample preparation and the applications for PMs in recent years.

关 键 词：膜蛋白 液质联用 蛋白组学 综述 

分 类 号：R1[医药卫生—公共卫生与预防医学]