基于气质联用的保元汤抗缺氧作用代谢组学研究  

Subacute Metabonomics of Baoyuan Decoction Based on GC-MS

作　　者：杨茜 黄荣清 肖炳坤 杨建云 Yang Qian;Huang Rongqing;Xiao Bingkun;Yang Jianyun(Institude of Radiation Medicine,Academy of Military Medical Sciences,Beijing 100850,China)

机构地区：[1]军事医学研究院放射与辐射医学研究所,北京100850

出　　处：《世界科学技术-中医药现代化》2018年第8期1453-1458,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：北京市科学技术委员会北京市自然科学基金面上基金项目(7172161):金铃子散复方配伍的代谢组学研究;负责人:黄荣清

摘　　要：目的:通过气相色谱-质谱联用技术对中药名方保元汤的抗缺氧作用及途径进行研究,寻找生物标记物,阐明代谢通路。方法:小鼠随机分为6组(正常组、模型组、对照组和保元汤低、中、高3个剂量实验组),每组8只。灌胃给药12天后,将小鼠放入密闭具塞广口瓶中,以死亡时间作为抗缺氧能力指标。收集血液进行血常规分析和气相色谱质谱联用仪分析。数据通过软件分析寻找差异性代谢物,并对代谢通路进行分析。结果:保元汤各剂量组抗缺氧死亡时间比较模型组均有明显延长,代谢组学分析中共找到25个内源性标志物。结论:保元汤具有显著性抗缺氧作用,通过代谢组学分析找到4条代谢通路,包括(1)甘氨酸、丝氨酸和苏氨酸代谢路径,(2)氨酰-tRNA合成通路,(3)缬氨酸、亮氨酸和异亮氨酸代谢路径,(4)β-丙氨酸代谢路径。This paper was aimed toindentify metabolic biomarkers and metabolic pathways underlie the anti-anoxia effect of traditional Chinese medicine Baoyuan Decoction by gas chromatography-mass spectrometry(GC-MS).Mice were randomly divided into 6 groups(normal group,model group,control group,and low,middle,and high dose of three experimental groups),with 8 mice in each group.After 12 days of intragastric administration,the mice were placed in closed stoppered jars to moniter time.The time of death was used as an indicator of anti-anoxia effect,and the serum data was extracted by GC-MS.The data was analyzed by software to finding differential metabolites and analyzing metabolic pathways.The duration of anti-anoxia effect was significantly prolonged in each dose of Baoyuan Decoction,and 25 metabolic biomarkers were found in the metabolomic analysis.Conclusion: Baoyuan Decoction has significant anti-hypoxic effect,and 4 metabolic pathways were found through metabolomic analysis,including Glycine,serine and threonine metabolism.Aminoacyl-tRNA biosynthesis.Valine,leucine and isoleucine biosynthesis 2,beta-Alanine metabolism.

关 键 词：保元汤 气相色谱-质谱联 代谢组学 常压抗缺氧模型 

分 类 号：R285.1[医药卫生—中药学]