受体色谱:中药靶向活性成分高效筛选技术  

Receptor Chromatography: High-efficient Screening Technology of Receptor-targeting Bioactive Compounds in Traditional Chinese Medicine

作　　者：刘嘉君 贾晓妮 王静[1] 曾凯竹 李倩[1] 赵新锋[1] Liu Jiajun;Jia Xiaoni;Wang Jing;Zeng Kaizhuo;Li Qian;Zhao Xinfeng(College of pharmacy,Northwest University,Xi'an 710069,China;Xi'an Mental Health Center,Xi'an 710100,China)

机构地区：[1]西北大学药学院,西安710069 [2]西安市精神卫所中心,西安710100

出　　处：《世界科学技术-中医药现代化》2018年第8期1476-1481,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委面上基金项目(21775119):基于G-蛋白偶联受体信号转导通路的复杂体系药物活性成分色谱筛选方法,负责人:赵新锋;国家自然科学基金委青年基金项目(21705126):内皮素受体A-药物相互作用快速分析受体色谱方法的建立与评价,负责人:李倩;国家教育部创新团队滚动支持(IRT_15R55):基于秦巴优势生物资源的生命效应分析与新药创制研究,负责人:郑晓晖.

摘　　要：中药活性成分高效筛选技术是化学、生物学和医药学等众多领域的前沿研究问题之一。笔者课题组将G-蛋白偶联受体(GPCRs)识别药物的高特异性和高效液相色谱(HPLC)的高分离能力相结合,提出了受体色谱概念,探索了多种受体固定化方法,建立了系列受体色谱模型,成功将其应用于药物-受体相互作用研究及中药活性成分筛选,为中药受体靶向活性成分高效筛选提供了新方法。The high-efficient screening technology of bioactive compounds in Traditional Chinese Medicine(TCM) is one of the main research issues in many fields,including chemistry,biology and medicine science.This paper defined the new concept of receptor chromatography by combining the high recognizing specificity between G-protein-coupled receptors(GPCRs) and their ligands with the high separation capacity of high performance liquid chromatography(HPLC).Subsequent studies were performed to pursue the methods for receptor immobilization and the establishments of desired receptor chromatographic models.These models were validated by the successful application in drug-receptor interaction analysis and the screening of bioactive compounds from traditional Chinese medicine.Receptor chromatography proved to be a new promising alternative for high-efficient screening receptor-targeting bioactive compounds from TCM.

关 键 词：活性成分筛选 G-蛋白偶联受体 受体色谱 药物-受体相互作用 

分 类 号：R285.5[医药卫生—中药学]