益气明目丸对视网膜色素变性大鼠视网膜BaxmRNA、Caspase-3mRNA表达的影响  

Effects of Yiqi Mingmu Pills on Retinal BaxmRNA Protein and Caspase-3mRNA Expression in Retinal Pigmented Rats

作　　者：蒋鹏飞[1,2] 王英 潘坤[1,2] 彭俊 徐剑[1,2] 彭清华 Jiang Pengfei;Wang Ying;Pan Kun;Peng Jun;Xu Jian;Peng Qinghua(Hunan University of Traditional Chinese Medicine,Changsha 410208,China;First Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410007,China;Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Eye,Ear,Nose and Throat Diseases in Hunan Province,Changsha 410208,China)

机构地区：[1]湖南中医药大学,长沙410208 [2]中医药防治眼耳鼻咽喉疾病湖南省重点实验室,长沙410208 [3]湖南中医药大学第一附属医院,长沙410007

出　　处：《世界科学技术-中医药现代化》2018年第10期1834-1839,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：湖南中医药大学中医诊断学国家重点学科开放基金优先资助项目(No.2015ZYZD02):基于Rho;XBPI表达探讨补虚活血法对虚中夹瘀证RP模型大鼠的干预研究;负责人:徐剑;彭清华;湖南省教育厅创新平台开放基金项目(16K065):虚活血法对RP虚中夹瘀证模型大鼠Rho;XBP1;Caspase12表达的研究;负责人:彭清华;湖南省科技厅中医药防治五官科疾病湖南省重点实验室建设项目(2017TP1018):中医药防治五官科疾病湖南省重点实验室;负责人:彭清华;长沙市科技局长沙市科技计划项目(kc1704005):中医药防治五官科疾病湖南省重点实验室;负责人:彭清华

摘　　要：目的:观察益气明目丸对RCS大鼠视网膜BaxmRNA、Caspase-3mRNA表达的影响。方法:24只RCS大鼠随机分为3组,分别为:空白组、模型组、益气明目丸组。空白组:RCS(rdy+/+,p+/+)大鼠共8只,雌雄各4只,灌胃生理盐水;模型组:RCS(rdy-/,p-/-)大鼠共8只,雌雄各4只,灌胃生理盐水;益气明目丸组:RCS(rdy-/,p-/-)大鼠共8只,雌雄各4只,灌胃益气明目丸悬浊溶液。灌胃30天后,HE染色观察视网膜各层结构,RT-qPCR法及Western Blot法分别测定各组视网膜组织中BaxmRNA、Caspase-3mRNA的表达水平。结果:HE染色显示:益气明目丸组大鼠视网膜厚度明显较模型组大鼠视网膜增厚,视网膜色素上皮条带部分可见,部分外核层光感受器感觉纤毛层部分可见,光感受器细胞核数目较模型组多,外从状层、外核层、视杆视锥层较模型组结构清晰且增厚,RT-qPCR检测显示:益气明目丸组BaxmRNA、Caspase-3mRNA相对表达量明显低于模型组,差异有显著统计学意义(P <0.01),WB检测显示:益气明目丸组Bax蛋白、Caspase-3蛋白相对表达量明显低于模型组,差异有统计学意义(P <0.01)。结论:益气明目丸对RP视网膜的超微结构具有保护作用;益气明目丸可抑制视网膜上BaxmRNA、Caspase-3mRNA的表达,进而减轻视网膜感光细胞的凋亡,达到保护视细胞的目的。Objective: To observe the effect of Yiqi Mingmu Pill on the expression of BaxmRNA protein and caspase-3 mRNA in retina of RCS rats. Methods: Twenty-four RCS rats were randomly divided into three groups: blank group,model group, Yiqi Mingmu Pill group. In the blank group, there were 8 RCS(rdy+/+,p+/+) rats, with 4 males and 4 females, and they received intragastric administration of normal saline. The model group consisted of 8 RCS(rdy-/, p-/-)rats, with 4 males and 4 females, and they received intragastric administration of normal saline. Yiqi Mingmu Pills groupconsisted of 8 RCS(rdy-/, p-/-) rats, with 4 males and 4 females, respectively. They received intragastric administrationof suspension of Yiqi Mingmu Pills. After intragastric administration for 30 days, the structure of retina layers wasobserved by HE staining. BaxmRNA and Caspase-3 mRNA in retinal tissues of each group were measured by RT-qPCRand Western Blot. Results: HE staining showed that the retina thickness of rats in Yiqi Mingmu Pill group wassignificantly thicker than that of the model group. The retinal pigment epithelial band was partially visible, and part of theouter nuclear layer photoreceptor sensory ciliary layer was visible. The number of photoreceptor cell nuclei was higherthan that of the model. There were many groups. The outer layer, outer core layer, and the visual cone layer were clearerand thicker than the model group. RT-qPCR showed that the relative expression of Bax mRNA and Caspase-3 mRNA inYiqi Mingmu Pills group was significantly lower than the model group, and the difference was statistically significant(P<0.01), WB test showed that the relative expression of Bax protein and Caspase-3 protein in Yiqi Mingmu Pill group wassignificantly lower than that in the model group, and the difference was statistically significant(P<0.01). Conclusion: YiqiMingmu Pill has protective effect on the ultrastructure of RP retina. Yiqi Mingmu Pills can reduce the apoptosis of retinalphotoreceptor cells by inhibiting the expression of BaxmRNA an

关 键 词：益气明目丸 RCS大鼠 BAXMRNA CASPASE-3MRNA 细胞凋亡 

分 类 号：R289.9[医药卫生—方剂学]