基于多成分和总鞣质测定的藏药大三果及其鞣质部位质量研究  

Comprehensive Evaluation of the Quality of Tibetan Medicine Triphala's Tannin Parts Based on There Characteristic Components and the Total Tannin Content

作　　者：叶婷 崔雅萍[1] 梁林金 梁文仪[1] 简平 周坤 吴玲芳[1] 李师[1] 亓旗 张兰珍[1] Ye Ting;Cui Yaping;Liang Linjin;Liang Wenyi;Jian Ping;Zhou Kun;Wu Lingfang;Li Shi;Qi Qi;Zhang Lanzhen(College of Chinese Materia Medica,Bering University of Chinese Medicine,Beijing 102488,China)

机构地区：[1]北京中医药大学中药学院,北京102488

出　　处：《世界科学技术-中医药现代化》2018年第9期1615-1621,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委面上基金项目(81274187):基于藏药余甘子酚酸类成分体内动态变化的抗癌药效物质与质量控制研究;负责人:张兰珍;北京中医药大学校级科研纵向发展基金(2016-ZXFZJJ-065):藏药大三果鞣质部位提取纯化质量控制及抗肿瘤活性研究;负责人:张兰珍

摘　　要：目的建立HPLC法测定大三果中3种特征成分含量,建立分光光度法测定大三果中总鞣质含量。方法采用Agilent ZORBAX SB-Aq(250×4.6 mm,5μm)色谱柱,流动相为甲醇-0.1%甲酸/水;流速为1 mL·min-1;检测波长为270 nm,测定大三果药材和鞣质部位没食子酸、柯里拉京和鞣花酸含量。以没食子酸为对照品,采用分光光度法测定大三果药材和鞣质部位总鞣质含量。结果 HPLC测定中没食子酸、柯里拉京和鞣花酸线性范围分别为0.91-4.55μg、0.274-1.368μg和0.329-2.634μg。药材中3种成分平均回收率依次分别为101.06%、101.72%和100.27%,鞣质部位中3种成分平均回收率分别为100.4%、100.85%和101.70%。分光光度法中没食子酸在1.008-10.08μg·mL-1成线性。药材和鞣质部位中总鞣质回收率分别为100.25%和100.52%。结论本方法快速、准确、重复性较好,可为大三果及其鞣质部位的质量控制提供依据。To determine there characteristic components content of Tibetan Medicine Triphala through establishing a HPLC method and its total tannin content through spectrophotometry. The chromatographic column of Agilent ZORBAX SB-Aq(250 × 4.6 mm, 5 μm) with methanol-0.1% formic acid/water as the mobile phase, the flow rate 1 mL/min, and the detection wavelength 270 nm was applied to determine the Gallic acid, colijing and ellagic acid content in medicinal materials and the tannin parts of Tibetan Medicine Triphala. With the gallic acid as control group, total tannin content of Triphala and its tannin parts was determined through spectrophotometry. It revealed in the HPLC test that the linear range of gallic acid, coracine and ellagic acid was 0.91-4.55 μg, 0.274-1.368 μg and 0.329-2.634 μg respectively. It also showed that the average recovery rates of the three components in the medicinal materials were 101.06%, 101.72%and 100.27% respectively. And the average recovery rates of the three components in the tannins were 100.4%, 100.85%and 101.70% respectively. The result of spectrophotometry showed that gallic acid was linear in1.008-10.08 μg·mL-1,and that the recovery rate of medicinal materials and tannin parts were 100.25% and 100.52% respectively. The method is rapid, accurate and repeatable, and it can provide basis for the quality control of Tribescens and its tannins.

关 键 词：大三果 鞣质部位 分光光度法 高效液相色谱法 含量测定 

分 类 号：R29[医药卫生—民族医学]