基于总鞣质和7种成分同时测定的诃子药材质量控制研究  

Study on Quality Evaluation of Terminalia Chebular Based on Simultaneous Determination of Total Tannin and 7 Components

作　　者：梁林金 亓旗 叶婷 梁文仪[1] 周坤 简平 张兰珍[1] Liang Linjin;Qi Qi;Ye Ting;Liang Wenyi;Jian Ping;Zhou Kun;Zhang Lanzhen(School of Chinese Materia Medica,Belting University of Chinese Medicine,Beijing 102488,China)

机构地区：[1]北京中医药大学中药学院,北京102488

出　　处：《世界科学技术-中医药现代化》2018年第9期1638-1644,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委面上基金项目(81274187):基于藏药余甘子酚酸类成分体内动态变化的抗癌药效物质与质量控制研究;负责人:张兰珍;北京中医药大学校级科研纵向发展基金(2016-ZXFZJJ-065):藏药大三果鞣质部位提取纯化质量控制及抗肿瘤活性研究;负责人:张兰珍

摘　　要：目的:建立分光光度法测定诃子药材总鞣质含量和HPLC法同时测定7种成分没食子酸、安石榴苷A、没食子酸甲酯、安石榴苷B、柯里拉京、五没食子酰葡萄糖和鞣花酸含量。方法:以没食子酸为对照品,采用磷钼钨酸比色法(药典法),测定总鞣质含量。采用色谱柱Agilent ZORBAX SB-Aq-C18(250×4.6 mm,5μm),流动相为磷酸水(0.1%)-甲醇,流速1 mL·min-1,柱温30℃,检测波长为270 nm,梯度洗脱,同时测定诃子药材中7种成分含量。结果:诃子总鞣质平均含量为30.31%,RSD为0.55%;平均加样回收率为99.70%,RSD为21.97%(n=6)。没食子酸、安石榴苷A、安石榴苷B、没食子酸甲酯、柯里拉京、五没食子酰葡萄糖和鞣花酸在各自范围线性良好,加样回收率在100.10%-102.77%。结论:建立诃子药材中总鞣质、没食子酸、安石榴苷A、安石榴苷B、没食子酸甲酯、柯里拉京、五没食子酰葡萄糖和鞣花酸含量测定方法简便,准确度高,专属性强,可用于诃子药材的质量控制。The spectrophotometric method was established for the determination of total tannin content in gardenia medicinal materials and tannins, and HPLC method was applied to simultaneously determine gallic acid, punical glucoside A, methyl gallate, punny glucoside B, corilagin, pentagalloyl glucose and the content of ellagic acid. Taking gallic acid as a reference substance, a phosphomolybdate tungstic acid colorimetric method(Pharmacopoeia method) was used to determine the total tannin content. With the chromatographic column Agilent ZORBAX SB-Aq-C18(250 × 4.6 mm, 5 microns), mobile phase for phosphoric acid(0.1%), methanol, water flow rate of 1 ml/min, column temperature 30℃,detection wavelength of 270 nm, gradient elution, 7 kinds of component contents in myrobalan medicinal materials were measured at the same time. The result showed that the average content of total tannins of Radix Scutellariae was 30.31%,RSD was 0.55%;the average recovery rate was 99.70%, and the RSD was 21.97%(n = 6). In the scorpion medicinal materials, gallic acid, punical glucoside A, punny glucoside B, methyl gallate, punny glucoside B, creatin, pentagalloglucose and ellagic acid have a good linear relationship in their respective ranges. The recovery rate of the sample is between100.10% and 102.77%. The establishment of the determination method of total tannin, gallic acid, punical glucoside,methyl gallate, corridain, pentagalloglucose and ellagic acid in the scorpion medicinal materials is simple and accurate,which has strong specificity and can be used for quality control of Terminalia chebular.

关 键 词：诃子 总鞣质 比色法 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]