基于化学计量学分析的诃子药材高效液相色谱指纹图谱研究  

Study on HPLC Fingerprint of Terminalia chebular Retz Based on Chemometrics Analysis

作　　者：梁文仪[1] 亓旗 梁林金 叶婷 周坤 简平 张兰珍[1] Liang Wenyi;Qi Qi;Liang Linjin;Ye Ting;Zhou Kun;Jian Ping;Zhang Lanzhen(Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学中药学院,北京102488

出　　处：《世界科学技术-中医药现代化》2018年第9期1645-1649,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委面上基金项目(81274187):基于藏药余甘子酚酸类成分体内动态变化的抗癌药效物质与质量控制研究;负责人:张兰珍;北京中医药大学校级科研纵向发展基金(2016-ZXFZJJ-065):藏药大三果鞣质部位提取纯化质量控制及抗肿瘤活性研究;负责人:张兰珍

摘　　要：目的:建立诃子药材HPLC指纹图谱。方法:安捷伦ZORBAX SB-Aq C18色谱柱(4.6×250 mm,5μm);流动相为0.1%磷酸水-甲醇,梯度洗脱,流速1 mL·min-1,柱温35℃,检测波长270 nm,进样量20μL。采用相似度软件和SPSS、SIMCA软件对10批诃子药材的色谱数据进行化学计量学分析。结果:标定17个共有峰,10批药材相似度在0.984-0.999之间,表明所含化学成分基本相同,差异不大。聚类分析可将样品分为4类,与主成分分析、偏最小二乘判别分析结果一致,VIP分析结果显示色谱峰5(没食子酸甲酯)、11、1(没食子酸)、10、12可能是鉴别诃子样品最主要的色谱峰。结论:所建立的诃子药材HPLC指纹图谱方法准确、稳定,通过化学计量学分析寻找区分不同品质诃子样品的标志性成分,可为诃子质量控制及评价提供参考。This paper was aimed to establish the HPLC fingerprint of Terminalia chebular Retz The analysis was performed on Agilent ZORBAX SB-Aq C18 column(4.6×250 mm, 5 μm). The mobile phase was 0.1% phosphate watermethanol solution with gradient elution. The flow rate was 1 mL·min-1. The temperature was maintained at 35℃. The detection wavelength was 270 nm. The injection volume was 20 μL. The chromatographic data of 10 samples was analyzed by similarity of chromatographic data, SPSS software and SIMCA software. The results showed that there were17 common peaks in the diagram. The similarity of 10 samples was between 0.984 and 0.999, which showed that there was no significant difference among these chemical components. The samples were classified into four types according to cluster analysis, which shared the similar results as the principal components analysis(PCA) and partial least sq uares analysis(PLS-DA). VIP analysis result showed that peak 5(methyl gallate), 11 and 1(gallic acid), 10, 12 may be the main chromatographic peaks to identify the samples. It was concluded that chemometrics analysis could be used to analyze, find and distinguish the marker ingredients of Terminalia chebular Retz. This method was accurate and stable,which can provide a reference for the quality control and evaluation of Terminalia chebular Retz.

关 键 词：诃子 指纹图谱 化学计量学 偏最小二乘判别分析 

分 类 号：R284.1[医药卫生—中药学]