中药复方消水方抑制小鼠恶性胸腔积液的形成及其机制研究  

Formation and mechanism of compound Chinese herbal medicine Xiaoshui Formula to inhibit malignant pleural effusion in mice

作　　者：金志超 席玉鹏 张兴 杨子禛 郭秋均[2] 花宝金[3] 施展[4] JIN Zhi-chao;XI Yu-peng;ZHANG Xing;YANG Zi-zhen;GUO Qiu-jun;HUA Bao-jin;SHI Zhan(Department of Oncology,Jiangsu Province Hospital of Traditional Chinese Medicine,Jiangsu 210029,China;Department of Oneology,Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053;Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053;Institute of Basic Research in Clinical Medicine,China Academy of Chinese Medical Sciences,Beijing 100700)

机构地区：[1]江苏省中医院肿瘤科,江苏210029 [2]中国中医科学院广安门医院肿瘤科,北京100053 [3]中国中医科学院广安门医院,北京100053 [4]中国中医科学院中医临床基础医学研究所,北京100700

出　　处：《北京中医药》2018年第12期1129-1134,F0003,共7页Beijing Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81603610);"十二五"国家科技支撑计划(2014 BAI10B01)

摘　　要：目的在动物层面验证消水方对恶性胸腔积液的抑制作用,并探讨其机制。方法建立小鼠恶性胸腔积液(MPE)模型,通过小动物活体成像、Luminex及免疫组化等方法观察消水方对小鼠MPE、小鼠生存期、胸膜转移瘤、胸膜通透性及血管新生、胸水血管内皮生长因子(VEGF)的作用。结果消水方组小鼠MPE体积明显减少,生存期延长;消水方组与对照组对胸膜转移瘤的作用无显著差异;消水方组胸水与外周血依文思蓝浓度比值低于对照组,胸水VEGF表达水平低于对照组,胸膜肿瘤CD31及CD34表达也低于模型对照组,差异均具有统计学意义。结论消水方可以抑制小鼠MPE生成,其作用机制可能与降低胸膜通透性、抑制血管生成有关。Objective To verify the inhibitory effect of Xiaoshui Formula(XSF)on malignant pleural effusion(MPE)in animal level and explore its mechanism.Methods After MPE mice model was established,the volume of MPE,survival time,pleural metastasis,pleural permeability and angiogenesis,vascular endothelial growth factor(VEGF)expression were assessed using bioluminescence imaging,Luminex and immunohistochemistry.Results The MPE volume of mice in XSF group was significantly reduced and the survival time was prolonged.There was no significant difference between the XSF group and the control group in pleural metastases.The Evans’blue ratio of MPE to peripheral serum,the level of VEGF in MPE,and the expression of CD31 and CD34 in pleural tumors were much lowered in XSF group than that of the control group all with statistically significant difference.Conclusion Xiaoshui Formula can inhibit the production of MPE in mice,and its mechanism may be related to reducing pleural permeability and inhibiting angiogenesis.

关 键 词：恶性胸腔积液 中药 消水方 血管新生 胸膜通透性 

分 类 号：R285.5[医药卫生—中药学]