急性肺栓塞患者血清miR-1233水平检测及临床意义  

Detection of serum miR-1233 level in patients with acute pulmonary embolism and its clinical significance

作　　者：张奇[1] 沈观樵[1] 赵晓飞[2] 童海江[1] ZHANG Qi;SHEN Guan-qiao;ZHAO Xiao-fei;TONG Hal-jiang(Clinical Laboratory,Shaoxing Second Hospital,Shaoxing,Zhefiang 312000,China)

机构地区：[1]绍兴第二医院检验中心,浙江绍兴312000 [2]杭州师范大学附属医院检验科,浙江杭州310015

出　　处：《中国卫生检验杂志》2019年第3期307-309,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的探讨急性肺栓塞患者血清miR-1233水平和危险因素分层之间的关系。方法收集2016年7月-2017年12月急性肺栓塞患者及对照者的血清,应用miRNA表达谱芯片进行筛选,在50例急性肺栓塞患者及25例对照者的血清中进行实时荧光定量聚合酶链反应(real-time fluorescent quantitative polymerase chain reaction,RTFQ-PCR)验证,分析miR-1233标志物与急性肺栓塞危险因素分层关系及其诊断价值。结果对6例急性肺栓塞和6例对照者的血清miRNA表达谱分析,筛选出10个miRNA。根据筛选出的血清miRNA再对50例急性肺栓塞患者进行验证,结果显示,miR-1233、miR-197、miR-126和miR-208b在所有血液标本中均能检测出Ct值,miR-1233、miR-197和miR-126在急性肺栓塞患者血清中表达水平较对照者高(P<0.05),而miR-208b在对照者血清中表达水平较急性肺栓塞高(P<0.05)。血清miR-1233随着危险因素分层上升而升高(P<0.05)。当设定界值定在5.6时,miR-1233对急性肺栓塞的诊断灵敏度为73.1%,特异度为82.6%,AUC为0.864 (P<0.05)。结论血清miR-1233升高与急性肺栓塞危险因素分层关系密切,在诊断和预测急性肺栓塞及疗效观察中具有潜在的临床意义。Objective To explore the relationship between serum miR-1233 level and stratification of risk factors in patients with acute pulmonary embolism. Methods Sera from patients with acute pulmonary embolism and healthy controls from July 2016 to December 2017 were collected and screened using miRNA expression profiling chips. Real-time fluorescent quantitative polymerase chain reaction(RTFQ-PCR) was performed to verify the relationship between miR-1233 markers and risk factors of acute pulmonary embolism and to discuss its diagnostic value. Results miR-1233, miR-197, miR-126 and miR-208 b were able to detect Ct values in all blood samples. The levels of miR-1233, miR-197 and miR-126 in serum of patients with acute pulmonary embolism were higher than that of healthy controls(P<0.05). The serum level of miR-208 b in the control group was higher than that of the patients with acute pulmonary embolism(P<0.05). Serum miR-1233 increased with the rise of risk factor stratification(P<0.05). When the cut-off value was set at 5.6, the diagnostic sensitivity of miR-1233 for acute pulmonary embolism was 73.1%, specificity was 82.6%, and AUC was 0.864(P<0.05). Conclusion The increase of serum miR-1233 is closely related to risk factors stratification of acute pulmonary embolism. It is a potential value in the diagnosis and prediction of acute pulmonary embolism.

关 键 词：血清miR-1233 急性肺栓塞 危险因素分层 

分 类 号：R563.5[医药卫生—呼吸系统]