历年生鲜乳中舒巴坦敏感β-内酰胺酶类药物检验考核及注意事项分析  

Inspection and key points for testing of Sulbactam sensitive β-lactamases in raw milk over the years

作　　者：狄彩霞[1,2] 杨永青 莎娜 栗艳芳 李秀萍 李宝贺[1,2] 任超 骆洪 DI Caixia;YANG Yongqing;Shana;LI Yanfang;LI Xiuping;LI Baohe;REN Chao;LUO Hong(Inspection and Test Center of Quality and Safety Supervision of Agricultural Products,Ministry of Agriculture,Huhhot 010031,China;Inner Mongolia Third-party Center of Inspection and Testing For Milk Quality and Safety,Huhhot 010031,China)

机构地区：[1]农业部农产品质量安全监督检验测试中心,内蒙古呼和浩特010031 [2]内蒙古自治区生鲜乳质量安全第三方检验测试中心,内蒙古呼和浩特010031

出　　处：《畜牧与兽医》2019年第1期131-135,共5页Animal Husbandry & Veterinary Medicine

基　　金：农业基础性长期性监测(ZX03S0501)

摘　　要：β-内酰胺酶非法掺入生鲜乳可分解其中残留β-内酰胺类抗生素(青霉素、头孢菌素),使常规检测方法无法检出有抗奶。为检测奶牛是否用过β-内酰胺类抗生素,检测部门开展了β-内酰胺酶类药物检测工作;而为了提高质检单位对乳及乳制品中β-内酰胺酶类项目检测结果的准确性及检测技术水平,农业部畜牧业司开展了对有资质的质量检测中心β-内酰胺酶类检测项目考核。本文即对历年考核结果进行归纳总结,同时对检测中需要注意事项进行分析阐述,以期为今后质量检测单位顺利通过考核及其他性能实验室准确检测乳及乳制品中舒巴坦敏感β-内酰胺酶类药物打下基础。β-lactamases is an illegal additive to raw milk which breaks down β-lactam antibiotics (penicillins and cephalosporins),so that antibiotics contained in milk cannot be detected by conventional detection methods. In order to detect whether dairy cows have been administered with β-lactam antibiotics,inspection and test centers perform the β-lactamase test to confirm safety of milk. The Animal Husbandry Department of Ministry of Agriculture have organized inspections of the β-lactamase testing project to improve the accuracy and the level of β-lactamase testing in milk and other dairy products. This article summarizes and analyses the results of the β-lactam tests performed over the last seven years,especially proposing related key points and notes to be careful with,which may be helpful for related laboratories to improve accuracy of β-lactamase test.

关 键 词：Β-内酰胺类抗生素 Β-内酰胺酶 舒巴坦 杯碟法 

分 类 号：S851.34[农业科学—预防兽医学]