四味消瘤饮抗脑胶质瘤血管生成的研究  

Study on the Inhibition of Siwei Xiaoliu Yin on Angiogenesis in Gliomas

作　　者：詹文刚 张志强[1] 谢才军[1] 谭齐家[1] 李聪[1] 陈雲 陈慧靖 ZHAN Wengang;ZHANG Zhiqiang;XIE Caijun;Tan Qijia;LI Cong;CHEN Yun;CHEN Huijing

机构地区：[1]广州中医药大学,广东广州510405

出　　处：《新中医》2019年第1期9-12,共4页New Chinese Medicine

基　　金：广东省科技厅-社会发展领域项目(412030198018)

摘　　要：目的:观察四味消瘤饮对脑胶质瘤血管生成的抑制作用,探讨四味消瘤饮抑制脑胶质瘤血管生成的机制。方法:建立人脑胶质瘤细胞株U87皮下移植瘤模型,将裸鼠随机分为生理盐水组、常规剂量四味消瘤饮组、大剂量四味消瘤饮组、替莫唑胺(Temozolomide,TMZ)组、四味消瘤饮+TMZ组,测量裸鼠肿瘤体积,Weidner法计数裸鼠肿瘤组织微血管密度,酶联吸附法检测裸鼠血清血管内皮生长因子(Vascular endothelial growth factor,VEGF)、内皮抑素(Endostatin,ES)的含量。结果:与生理盐水组比较,TMZ组肿瘤体积减小(P <0.05,P <0.01);常规剂量四味消瘤饮组、大剂量四味消瘤饮组、四味消瘤饮+TMZ组肿瘤体积、组织微血管密度(Microvessel density,MVD)、血清VEGF含量减小,血清ES含量升高,差异均有统计学意义(P <0.05,P <0.01)。结论:四味消瘤饮能抑制脑胶质瘤血管生成,其机制可能是通过上调ES和下调VEGF实现的。Objective:To observe the inhibitory effect of Siwei Xiaoliu Yin on angiogenesis in gliomas,and explore its mechanism.Methods:Established subcutaneou transplantation tumor model of U87 glioma,randomly divided nude mice into the normal saline group,the routine dose of Siwei Xiaoliu Yin group,the high dose of Siwei Xiaoliu Yin group,the temozolomide(TMZ)group,and the Siwei Xiaoliu Yin plus TMZ group,measured the tumor volume,counted the microvessel density(MVD)of tumor tissues by Weidner method,and detected the content of vascular endothelial growth factor(VEGF)and endostatin(ES)in serum of nude mice by enzyme-linked immunosorbent assay(ELISA).Results:Compared with that in the normal saline group,the tumor volume in the TMZ group was decreased(P<0.05).The tumor volume,MVD and the content of VEGF in serum in the routine dose and the high dose of Siwei Xiaoliu Yin groups and the Siwei Xiaoliu Yin plus TMZ group were decreased,while the content of ES in the serum was increased when compared with those in the normal saline group,differences being significant(P<0.05).Conclusion:Siwei Xiaoliu Yin can inhibit the angiogenesis in gliomas,and the mechanism is probably to up-regulate endostatin and to down-regulate vascular endothelial growth factor.

关 键 词：四味消瘤饮 胶质瘤 血管生成 动物实验 裸鼠 

分 类 号：R285.5[医药卫生—中药学]