白藜芦醇通过Nrf2途径发挥抗冠状动脉粥样硬化及心肌细胞保护作用  

Anti-Coronary Atherosclerosis and Cardioprotective Effect of Resveratrol by Regulating Nrf2 Signal Pathway

作　　者：朱华芳 毛承誉 曾华甦 高霖 顾俊[2] 王长谦[2] Zhu Huafang;Mao Chengyu;Zeng Huasu;Gao Lin;Gu Jun;Wang Changqian(Department of Internal Medicine,Geriartic Nursing Hospital of Huangpu District,Shanghai 200010,China;Department of Cardiology,the Ninth People's Hospital of Medical College of Shanghai Jiao Tong University)

机构地区：[1]上海市黄浦区老年护理医院内科,上海200010 [2]上海市交通大学附属第九人民医院心内科

出　　处：《中国药师》2019年第1期10-14,共5页China Pharmacist

基　　金：国家自然科学基金项目(编号:81470546)

摘　　要：目的:探讨白藜芦醇通过Nrf2途径发挥抗冠状动脉粥样硬化及心肌细胞保护作用。方法:将50小鼠按随机数字表法分为5组:空白对照组、模型组、白藜芦醇高(150 mg·kg^(-1)·d^(-1))、中(100 mg·kg^(-1)·d^(-1))、低(50 mg·kg^(-1)·d^(-1))剂量组,每组10只。除空白对照组正常喂养外,其余各组均给予高脂饲料喂养。各给药组灌胃给药,体积为0. 4 ml,1次/d,空白对照组和模型组给予等体积生理盐水。每周测量体质量,13周后比较小鼠血脂水平、主动脉及心肌组织改变、Nrf2表达情况等。结果:与空白对照组比较,模型组小鼠从第3周开始体质量较空白对照组显著增加(P <0. 05);血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)水平显著升高,高密度脂蛋白(HDL)水平显著降低(P <0. 05); Nrf2表达差异无统计学意义(P> 0. 05);小鼠动脉内膜斑块面积增多,厚度增大;心肌间隙扩大,细胞排列紊乱,心肌横纹消失。与模型组比较,白藜芦醇高(第3周开始)、中、低(第9周开始)剂量组小鼠的体质量显著降低(P <0. 05);白藜芦醇各剂量组小鼠血清中TC、TG、LDL水平均显著降低,HDL水平和Nrf2表达显著升高(P <0. 05);以上作用均呈剂量依赖性。白藜芦醇组小鼠的病理组织情况与模型组相比得到改善。结论:白藜芦醇能通过Nrf2途径有效发挥抗动脉粥样硬化及心肌保护作用。Objective:To investigate the anti-coronary atherosclerosis and cardioprotective effect of resveratrol by regulating Nrf2 signal pathway.Methods:Totally 50 mice were randomly divided into 5 groups:the blank control group,the model group,resveratrol high(150 mg·kg^-1·d^-1),medium(100 mg·kg^-1·d^-1)and low(50 mg·kg^-1·d^-1)dosage groups with 10 ones in each.Except for the normal feeding in the blank control group,all the other groups were fed with high-fat diet.All the groups were with intragastric administration,0.4 ml,once a day,and the blank control group and the model group were given saline with the same volume.The body weight was measured weekly.The blood lipid levels,changes of aortic and myocardial tissue,and Nrf2 expression were tested after 13 weeks.Results:Compared with that in the blank control group,the body weight of mice in the model group increased significantly from the third week(P<0.05).The serum levels of TC,TG and LDL in the model group increased significantly,while HDL level decreased significantly(P<0.05),and the difference in the Nrf2 expression was not statistically significant(P>0.05).The plaque and thickness of area in the model group increased.The myocardial space expanded,the cells were arranged in disorder,and the myocardial striations disappeared.Compared with that in the model group,the body weight of mice in resveratrol at high(from the third week),medium and low(from the ninth week)doses decreased significantly(P<0.05),the serum levels of TC,TG and LDL were significantly decreased,and the levels of HDL and Nrf2 were significantly increased(P<0.05).All the above effects were dosedependent.The pathological structure of mice in resveratrol groups was improved when compared with that in the model group.Conclusion:Resveratrol can play a role in anti-coronary atherosclerosis and cardioprotective effect through the Nrf2 pathway.

关 键 词：白藜芦醇 Nrf2途径 冠状动脉粥样硬化 心肌细胞 

分 类 号：R965.1[医药卫生—药理学]