Analysis of the binding sites with NL-101 to amino acids and peptides by HPLC/MS/MS  

作　　者：Lingzi Dai Nian Guo Yaqin Liu Shanshan Shen Qiufu Ge Yuanjiang Pan 

机构地区：[1]Department of Chemistry, Zhejiang University [2]Hangzhou Pharmaceutical Group Co., Ltd.

出　　处：《Chinese Chemical Letters》2019年第1期103-106,共4页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos. 21327010, 21372199)

摘　　要：The binding between NL-101, a novel nitrogen mustard anti-cancer drug, with amino acids and peptides has been investigated by high performance liquid chromatography electrospray tandem mass spectrometry(HPLC/ESI-MS/MS). This study offers supporting data of the interaction among drug and amino acids and peptides, which could potentially explain the cytotoxic and mutagenic effects of the drug. Collision-induced dissociation(CID) experiment demonstrated that under the same collision energy, the amino group combined with NL-101 adducts are sensitive and often produce more fragment ions; the carboxyl group combined with NL-101 adducts are hard to break and display fewer fragment ions. In addition, when other group(like sulfhydryl group) of amino acids binds to NL-101, CID spectra show different fragmentation pattern. These differences could display structural information about the drug adducts and be utilized as location of the authentic binding sites.The binding between NL-101, a novel nitrogen mustard anti-cancer drug, with amino acids and peptides has been investigated by high performance liquid chromatography electrospray tandem mass spectrometry(HPLC/ESI-MS/MS). This study offers supporting data of the interaction among drug and amino acids and peptides, which could potentially explain the cytotoxic and mutagenic effects of the drug. Collision-induced dissociation(CID) experiment demonstrated that under the same collision energy, the amino group combined with NL-101 adducts are sensitive and often produce more fragment ions; the carboxyl group combined with NL-101 adducts are hard to break and display fewer fragment ions. In addition, when other group(like sulfhydryl group) of amino acids binds to NL-101, CID spectra show different fragmentation pattern. These differences could display structural information about the drug adducts and be utilized as location of the authentic binding sites.

关 键 词：High-performance liquid chromatography/ tandem mass SPECTROMETRY NL-101 AMINO ACIDS PEPTIDES Binding sites 

分 类 号：O6[理学—化学]