An Automatic Solid Phase Extraction and Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry for Determination of Seven Microcystins at Ultra-Trace Levels in Surface Water  

作　　者：SHEN Fei XU Yanjuan WANG Ye CHEN Jing 

机构地区：[1]School of Environmental and Civil Engineering,Jiangnan University [2]Wuxi Environmental Monitoring Central Station

出　　处：《Wuhan University Journal of Natural Sciences》2019年第1期30-36,共7页武汉大学学报（自然科学英文版）

基　　金：Supported by the Environmental Monitoring and Research Foundation of Jiangsu Province(1621);the Taihu Lake Pollution Prevention and Control Project of Jiangsu Province(TH2016301);Postgraduate Research and Practice Innovation Program of Jiangsu Province in 2016(KYLX16_0813)

摘　　要：A method was developed for the detection of seven microcystins(microcystin-LR, RR, YR, LA, LY, LW and LF) in surface water using automatic solid-phase extraction(A-SPE) coupled with ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS). The automated solid-phase extraction system was used to extract microcystins(MCs) from water samples. UPLC-MS/MS was used to determine MCs concentrations in just 5 min. Method detection limits were from 0.3 to 0.9 ng/L, microcystin recoveries ranged from 83.8% to 114%, and the relative standard deviation(RSD) varied from 5.6% to 12.5%. This analytical approach was found to be simple, highly sensitive, accurate, which required little manual operation. Additionally, to validate this analytical method, A-SPE+UPLC-MS/MS was applied to characterize the concentration of MCs in Taihu Lake, Wuxi, China.A method was developed for the detection of seven microcystins(microcystin-LR, RR, YR, LA, LY, LW and LF) in surface water using automatic solid-phase extraction(A-SPE) coupled with ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS). The automated solid-phase extraction system was used to extract microcystins(MCs) from water samples. UPLC-MS/MS was used to determine MCs concentrations in just 5 min. Method detection limits were from 0.3 to 0.9 ng/L, microcystin recoveries ranged from 83.8% to 114%, and the relative standard deviation(RSD) varied from 5.6% to 12.5%. This analytical approach was found to be simple, highly sensitive, accurate, which required little manual operation. Additionally, to validate this analytical method, A-SPE+UPLC-MS/MS was applied to characterize the concentration of MCs in Taihu Lake, Wuxi, China.

关 键 词：MICROCYSTINS AUTOMATIC SOLID-PHASE extraction ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS) 

分 类 号：O656.3[理学—分析化学]