HIV-1 06044包膜糖蛋白gp140三聚体免疫原性分析  

Immunogenicity analysis of HIV-1 06044 gp140 trimer envelope

作　　者：许颖出 王甲业[1] 李妍[1] 卢亚楠 袁丽 庄敏[1] 凌虹[1] Xu Yingchu;Wang Jiaye;Li Yan;Lu Yanan;Yuan Li;Zhuang Min;Ling Hong(Department of Microbiology,Harbin Medical University,Heilongjiang Provincial Key Laboratory of Infection and Immuunity,Key Laboratory for Biology of Pathogens,Harbin 150081,China)

机构地区：[1]哈尔滨医科大学微生物学教研室、黑龙江感染与免疫重点实验室、黑龙江普通高校病原生物学重点实验室,150081

出　　处：《国际免疫学杂志》2019年第1期7-11,共5页International Journal of Immunology

基　　金：国家自然科学基金(81271824).

摘　　要：目的研究来源于一个Ⅰ型人类免疫缺陷病毒(human immunodeficiency virus type-1,HIV-1)慢性感染的广谱中和者包膜蛋白的免疫原性。方法用HIV-1 06044包膜三聚体gp140T蛋白分别免疫Balb/c小鼠和新西兰白兔。采用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)检测免疫兔血清特异性结合抗体滴度,用TZM-bl假病毒中和实验检测兔血清特异性中和抗体滴度及免疫小鼠脾细胞分泌细胞因子。结果家兔免疫血清呈较好的结合广度,与SF162 gp140、JRCSF gp120、Bal gp120广泛结合,末次免疫后终点滴度分别为83176.38±1.35、186208.71±2.57。兔血清在第三次免疫后即有较高滴度的特异性中和抗体,第3、4、5、6次免疫后两周,对SF162假病毒的中和抗体滴度为分别177、183、265和252。在末次免疫后14天的小鼠脾细胞中,白细胞介素(interleukin,IL)-2、IL-4、IL-6、IL-13,干扰素(interferon,IFN)-γ在免疫组中显著高表达(P值分别为0.0015、0.0009、0.0117、0.0001、0.0051)。结论06044 gp140T三聚体包膜蛋白有较好的免疫原性,并能较早地诱导较高水平的特异性中和抗体。Objective To study the immunogenicity of an envelope protein derived from a chronic in-fection with human immunodeficiency virus type Ⅰ (HIV-1). Methods Balb/ c mice and New Zealand white rabbits were immunized with 06044 gp140T. The specific binding antibody titers of the immunized rabbit serum were determined by enzyme-linked immunosorbent assay (ELISA),and the specific neutralizing antibody titer of rabbit serum was determined by TZM-bl pseudovirus neutralization assay. The cytokine secretion by the spleen cells of immunized mince was determined. Results Rabbit immune sera showed broadly binding breadth and endpoint titers to SF162 gp140,JRCSF gp120 and Bal gp120 were 83176. 38 ± 1. 35、186208. 71 ± 2. 57 respec-tively. After the third immunization,rabbits had high titer of specific neutralizing antibodies. The neutralizing an-tibody titer against SF162 pseudovirusat at two week after the 3rd,4th,5th,6th immunization was 177,183,265, 252 respectively. Interleukin(IL)-2,IL-4,IL-6,IL-13 and interferon(IFN)-γ significantly highly expressed in immunized mice than that in the control group at day 14 after the last immunization(P values were 0. 0015, 0. 0009,0. 0117,0. 0001,0. 0051 respectively). Conclusion 06044 gp140T trimeric envelope protein has good immunogenicity and can induce high specific antibody levels after early immunizations.

关 键 词：人类免疫缺陷病毒Ⅰ型 gp140 三聚体 特异性抗体 

分 类 号：R392[医药卫生—免疫学]