电针干预对失神经肌萎缩大鼠肌卫星细胞分化及肌纤维类型转化的影响  

Effect of electroacupuncture on amyotrophia and expression of myogenic differentiation-related genes of gastrocnemius in rats with chronic constriction injury of sciatic nerve

作　　者：赵丹丹[1,2] 唐成林 黄思琴[1,2] 罗翱 张安宁[1,2] 吴梦佳 安荟羽[1,2] 谭程方 邱丽[1,2] ZHAO Dan-dan;TANG Cheng-lin;HUANG Si-qin;LUO Ao;ZHANG An-ning;WU Meng-jia;AN Hui-yu;TAN Cheng-fang;QIU Li(College of Traditional Chinese Medicine,Chongqing Medical University,Chongqing 400016,China;Chongqing Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Metabolic Diseases,Chongqing 400016,China)

机构地区：[1]重庆医科大学中医药学院 [2]中医药防治代谢性疾病重庆市重点实验室,重庆400016

出　　处：《针刺研究》2019年第1期37-42,共6页Acupuncture Research

基　　金：国家自然科学基金(No.81273870);重庆市卫计委中医科研项目(No.zy20150249)

摘　　要：目的:观察电针干预对失神经肌萎缩大鼠腓肠肌中配对盒转录因子7(Pax7)、成肌分化抗原(Myod1)、肌细胞生成素(Myog)、肌球蛋白重链-Ⅱa(Myh2)、肌球蛋白重链-Ⅱx(Myh1)及肌球蛋白重链-Ⅰ(Myh7)表达的影响,探讨电针延缓失神经肌萎缩发展的可能机制。方法:将66只雄性SD大鼠随机分为假手术组24只、模型组24只和电针组18只。采用慢性坐骨神经压迫损伤制备大鼠右后肢失神经肌萎缩模型。造模1周后电针组取大鼠术侧"足三里""环跳"穴予电针治疗,每次10min,每日1次,每周6次,分别连续干预1、2、4周。称重计算各组大鼠的腓肠肌湿重比,HE染色测定腓肠肌纤维截面积及直径;实时荧光定量PCR检测造模第3周各组大鼠腓肠肌中Pax7、Myod1、Myog、Myh2、Myh1和Myh7mRNA的相对表达量。结果:与假手术组相比,造模1周后各时间点模型组大鼠腓肠肌湿重比显著降低(P<0.05)。模型组和电针组腓肠肌湿重比差异无统计学意义(P>0.05)。与假手术组相比,各时间点模型组大鼠术侧腓肠肌纤维截面积及直径显著减小(P<0.05);与模型组比较,各时间点电针组大鼠术侧腓肠肌纤维截面积及直径显著升高(P<0.05)。造模3周时,与假手术组相比,模型组大鼠术侧腓肠肌中Myod1和Myog mRNA表达量明显升高(P<0.01),而Myh2、Myh1和Myh7mRNA表达量明显降低(P<0.05,P<0.01);与模型组比较,电针干预则能有效上调Myod1、Myog和Myh7mRNA表达量(P<0.05,P<0.01);3组间Pax7mRNA表达量差异无统计学意义(P>0.05)。结论:电针干预大鼠"足三里""环跳"穴可能通过调控Myod1、Myog、Myh7mRNA的表达,影响肌卫星细胞的成肌分化水平及肌纤维类型的转换,延缓腓肠肌失神经肌萎缩的发展。Objective To explore the effect of electroacupuncture(EA)on amyotrophia and expression of paired box7(Pax7),myogenic differentiation antigen-1(Myod1),myogenin(Myog),myosin heavy chain-Ⅱa(Myh2),myosin heavy chain-Ⅱx(Myh1)and myosin heavy chain-Ⅰ(Myh7)of denervated gastrocnemius in rats with chronic constriction injury(CCI)of the right sciatic nerve,so as to explore its mechanisms underlying postponing development of amyotrophia.Methods Sixty-six SD adult male rats were randomly divided into sham operation(sham)group(n=24),model group(n=24)and EA group(n=18).The denervated muscle(gastrocnemius)atrophy model was established by CCI of the right sciatic nerve.EA(2 Hz,1.0 mA)was applied to the right"Zusanli"(ST36)and"Huantiao"(GB30)for 10 min,once a day,six times a week and for 1,2 and 4 weeks,respectively.After complete dissection of bilateral gastrocnemius muscles,their wet weight levels were measured and the ratio of wet weight(=that of the operation side/that of the non-operation side)was calculated,and the cross-sectional area(CSA)and diameter of the gastronemius were detected after fixation in 4%paraformaldehyde,sectioning,and H.E.staining.The expression levels of Pax7,Myod1,Myog,Myh2,Myh1 and Myh7 mRNAs in the gastrocnemius tissue after 3 weeks of modeling were detected with quantitative real time-PCR(qPCR).Results After 1 week of modeling,the ratios of wet weight of gastrocnemius and the CSA and fiber diameter at the 2 nd,3 rd and 5 th week were significantly smaller in the model group than in the sham group(P<0.05).The expression levels of Myod1 and Myog mRNAs were significantly up-regulated(P<0.01),and those of Myh2,Myh1 and Myh7 considerably down-regulated in the model group compared with the sham group(P<0.05,P<0.01).No significant changes were found in the expression levels of Pax7 mRNA after modeling and EA intervention(P>0.05).Following EA intervention,the CSA and diameterof the gastronemius were obviously increased(P<0.05),and the expression levels of Myod1,Myog and Myh7 further markedly or remark

关 键 词：失神经肌萎缩 电针 肌卫星细胞分化 肌纤维类型转化 

分 类 号：R245.97[医药卫生—针灸推拿学]