扩心方对tnnt2a基因突变斑马鱼心脏保护作用的研究  

Study on cardioprotective effect of Kuoxinfang on tnnt2a mutant zebrafish

作　　者：赵慧[1] 刘恋 费飞 王蕾 周端[1] 王旭 王佑华[1] ZHAO Hui;LIU Lian;FEI Fei;WANG Lei;ZHOU Duan;WANG Xu;WANG Youhua(Department of Cardiology,Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China;Department of Cardiology,Children's Hospital of Fudan University,Shanghai 201102,China;Department of Biochemistry and Molecular Biology,School of Basic Medicine,Fudan University,Shanghai 200032,China)

机构地区：[1]上海中医药大学附属龙华医院心内科,上海200032 [2]复旦大学附属儿科医院心内科,上海201102 [3]复旦大学基础医学院生物化学与分子生物学系,上海200032

出　　处：《上海中医药杂志》2019年第2期72-76,共5页Shanghai Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81328025;81873264);上海市科委科研计划项目(17401901600)

摘　　要：目的观察扩心方对与扩张型心肌病相似表型的tnnt2a突变斑马鱼模型的心脏保护作用,并探讨其可能的作用机制。方法以正常野生型斑马鱼作为对照组,tnnt2a突变斑马鱼作为模型组,tnnt2a突变斑马鱼给予5μg/ml的扩心方进行干预作为扩心方组。研究扩心方对tnnt2a突变斑马鱼的作用,观察生存率、静脉窦-动脉球(SV-BA)距离、病理切片HE染色、ATP含量、凋亡相关基因bcl-2/bax的表达情况。结果与模型组比较,扩心方对tnnt2a突变斑马鱼的心脏具有保护作用,可以提高模型组斑马鱼的生存率,增加ATP的含量,增加bcl-2/bax基因的表达(P<0.05,P<0.01)。结论扩心方对于tnnt2a突变斑马鱼模型具有心脏保护作用,其机制可能与改善能量代谢和减少心肌细胞的凋亡有关。Objective To observe the cardioprotective effect of Kuoxinfang on tnnt2a mutant zebrafish model with similar phenotype to dilated cardiomyopathy and explore the possible mechanism of action.Methods The normal wild-type zebrafish were used in the control group,the tnnt2a mutant zebrafish were used in the model group and the tnnt2a mutant zebrafish that received Kuoxinfang 5μg/ml were used in the Kuoxinfang group.The effect of Kuoxinfang on tnnt2a mutant zebrafish was studied and the survival rate,sinus venosus-bulbus arteriosus(SV-BA)distance,HE staining of pathological sections,ATP content and expression of the apoptosis-related gene bcl-2/bax were observed.Results Compared to the model group,Kuoxinfang has protective effect on the heart of tnnt2a mutant zebrafish,as it improves the survival rate,increases the ATP content and upregulates expression of the bcl-2/bax gene in zebrafish of the model group(P<0.05,P<0.01).Conclusion Kuoxinfang shows cardioprotective effect in the tnnt2a mutant zebrafish model,and the mechanism may be associated with improvement in energy metabolism and reduction in apoptosis of cardiomyocytes.

关 键 词：扩张型心肌病 斑马鱼 扩心方 心脏保护 

分 类 号：R285.5[医药卫生—中药学]