多指标综合加权评分法优选宁神颗粒提取工艺研究  

Optimizing extraction process of Ningshen Granule by comprehensive evaluation of multiple mark

作　　者：张春燕[1] 张冰冰 路建饶[2] 叶玉妹[3] 叶颖[1] 张宏[1] 朱建勇[1] ZHANG Chunyan;ZHANG Bingbing;LU Jianrao;YE Yumei;YE Ying;ZHANG Hong;ZHU Jianyong(Central Laboratory,Seventh People's Hospital affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200137,China;Department of Nephrology,Seventh People's Hospital affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200137,China;Department of Traditional Medical Science,Seventh People's Hospital affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200137,China)

机构地区：[1]上海中医药大学附属第七人民医院中心实验室,上海200137 [2]上海中医药大学附属第七人民医院肾病科,上海200137 [3]上海中医药大学附属第七人民医院传统医学科,上海200137

出　　处：《上海中医药杂志》2019年第1期96-100,共5页Shanghai Journal of Traditional Chinese Medicine

基　　金：上海市科委科技创新行动计划项目(17401902200);上海市浦东新区中医药研发与创新专项(PDZYYFCX-201713);上海市浦东新区卫计委成果转化项目(PW2017F-3)

摘　　要：目的优选宁神颗粒的提取工艺。方法采用L9(34)正交设计,以2,3,5,4’-四羟基二苯乙烯苷-2-O-β-D-葡萄糖苷、柚皮苷、丹酚酸B的含量及出膏率的综合评分为指标,考察料液比、提取时间、提取次数对提取效果的影响,优选宁神颗粒的最佳提取工艺。结果根据综合评分的结果,优选出最佳提取工艺为加8倍水,提取3次,每次0.5 h。结论该工艺简单可行,重复性好。Objective To investigate the extraction process of Ningshen Granules.Methods Orthogonal test was used to investigate the effects of solidliquid ratio,extraction time,and times on extraction process.The extraction process was optimized by comprehensive evaluation indexes based on the content of 2,3,5,4’-tetrahydroxy stilbene-2-O-β-D-glucoside,naringin,salvianolic acid B,and the extraction yield.Results According to the result of comprehensive score,the optimal extraction conditions of this preparation were as follows:extracted three times with 8 times of water quantity,each for0.5 h.Conclusion This optimized process is simple,feasible,repeatable.

关 键 词：宁神颗粒 正交试验 二苯乙烯苷 柚皮苷 丹酚酸B 综合加权 

分 类 号：R284.2[医药卫生—中药学]