姜黄素类似物H8对db/db小鼠内脏脂肪代谢影响  

Effect of curcumin analogue H8 on visceral fat metabolism in db/db mice

作　　者：李玲玉 唐春银 韩杨 温以杰 仲崇琦 吴丹 石永萍 袁晓环 LI Ling-yu;TANG Chun-yin;HAN Yang(Heilongjiang Province Key Laboratory of Anti-Fibrosis Biotherapy,Mudanjiang Medical University,Mudanjiang,Heilongjiang Province 157000,China)

机构地区：[1]牡丹江医学院黑龙江省抗纤维化生物治疗重点实验室,黑龙江牡丹江157000 [2]牡丹江市卫生监督所

出　　处：《中国公共卫生》2019年第2期183-187,共5页Chinese Journal of Public Health

基　　金：2017年度黑龙江省省属高等学校基本科研业务费科研项目(2017-KYYWFMY-0651);牡丹江医学院研究生创新科研项目(2017YJSCX-01MY)

摘　　要：目的探讨姜黄素类似物H8对db/db小鼠(糖尿病模型)内脏脂肪代谢的影响及机制。方法将db/db小鼠随机分为模型组、阳性对照组(罗格列酮5 mg/kg),H8低、高剂量组(5、10 mg/kg);连续干预8周,称量小鼠体重和内脏脂肪重量,检测血液生化指标,取肾周脂肪组织石蜡包埋,HE染色观察内脏脂肪细胞,免疫荧光法检测内脏脂肪细胞F4/80表达水平;Real-time PCR法检测脂代谢合成酶(FAS)、乙酰辅酶A羧化酶1(ACC1)、固醇调节元件绑定蛋白(SREBP)mRNA表达水平,蛋白印记法检测FAS、ACC1、SREBP的蛋白表达。结果与模型组比较,低、高剂量H8组小鼠内脏脂肪系数[分别为(3.20±0.23)%、(2.93±0.75)%]明显降低、内脏脂肪细胞体积[分别为(304.7±24.8)、(331.3±18.0)明显缩小(均P<0.01),内脏脂肪细胞F4/80表达水平[分别为(0.39±0.06)、(0.43±0.04)]显著降低,脂肪合成相关基因FAS[(217.0±46.5)、(249.1±31.4)]、ACC1 [(54.9±5.4)、(50.4±5.3)]、 SREBP[(14.2±1.7)、(13.4±1.5)]mRNA表达水平均明显下降(均P<0.01),脂肪合成相关蛋白FAS[(0.69±0.03)、(0.41±0.06)]、ACC1[(0.11±0.02)、(0.07±0.01)]、SREBP[(0.36±0.05)、(0.41±0.06)]表达也显著降低(均P<0.01)。结论姜黄素类似物H8能够改善db/db小鼠内脏脂肪代谢。Objective To investigate the effect and mechanism of curcumin analogue H8 on visceral fat metabolism in db/db mice(diabetes model). Methods Totally 32 specific pathogen free 8-week db/db mice were randomly divided into a model group(with sodium carboxymethyl cellulose), a positive control group(with 5 mg/kg rosiglitone), and low and high dose(5, 10 mg/kg) curcumin H8 groups. The administrations were performed with gastric gavage once a day continuously for 8 weeks. At the end of the experiment, the body and visceral fat weight of the mice were weighed;the blood biochemical indexes were detected;the perirenal fat tissue was embedded in paraffin;the visceral fat cells were observed with hematoxylin eosin staining;and the expression level of F4/80 in visceral fat cells was detected with immunofluorescence.Real-time PCR was used to detect the expressions of lipid metabolism synthase(FAS), acetyl-CoA carboxylase 1(ACC1),and sterol regulatory element binding protein(SREBP) mRNA;the protein expression of FAS, ACC1 and SREBP were detected by Western blot. Results Compared with those in the model group, all indicators of visceral fat metabolism decreased significantly in the low and high dose curcumin H8 group(P< 0.01 for all);the indicators included visceral fat coefficient(3.20 ± 0.23% and 2.93 ± 0.75%),volume of visceral fat cells(304.7 ± 24.8 and 331.3 ± 18.0),F4/80 in visceral fat cells(0.39 ± 0.06 and 0.43 ± 0.04),FAS mRNA(217.0 ± 46.5 and 249.1 ± 31.4),ACClmRNA(54.9 ± 5.4 and 50.4 ±5.3), SREBP mRNA(14.2±1.7 and 13.4 ± 1.5), FAS protein(0.69 ± 0.03 and 0.41 ± 0.06),ACC1 protein(0.11 ± 0.02 and0.07 ± 0.01),and SREBP protein(0.36 ± 0.05 and 0.41± 0.06). Conclusion Curcumin analog H8 can improve visceral fat metabolism in db/db mice.

关 键 词：糖尿病 脂代谢紊乱 脂肪合成酶 

分 类 号：R825.8[医药卫生—临床医学]