清心神安方对失眠大鼠脑组织神经递质含量的影响  

Effect of Qingxin Shenan Decoction on the Content of Neurotransmitters in Brain of Insomnia Rats

作　　者：凌燕[1] 冼绍祥[1] 陈汉裕 段骄 闫翠 周政[1] LING Yan;XIAN Shaoxiang;CHEN Hanyu;DUAN Jiao;YAN Cui;ZHOU Zheng(The First Affiliated Hospital of Guangzhou University of Chinese Medicine,State Key Laboratory of Chinese Internal Medicine,Guangzhou 510405 Guangdong,China)

机构地区：[1]广州中医药大学第一附属医院,广州中医药大学国家重点学科中医内科学实验室,广东广州510405

出　　处：《中药新药与临床药理》2018年第1期23-26,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省高等学校人才引进专项资金(粤财教[2013]246);广东省中医药局科研课题(20161125);2014年广东省科技计划项目(项目号2014A020221108).

摘　　要：目的观察清心神安方对失眠大鼠脑组织神经递质的影响。方法雄性SD大鼠随机取10只为空白组,其余为对氯苯丙氨酸(PCPA,300 mg·kg^(-1))造模组,造模2 d后随机分为模型组,清心神安方高、中、低(32,16,8 g·kg^(-1))剂量组和安定组(0.5 mg·kg^(-1)),连续灌胃给药10 d。使用高效液相色谱法检测海马组织谷氨酸(Glu)、γ-氨基丁酸(GABA)含量;免疫组化法检测海马组织5-羟色胺(5-HT)含量;ELISA法检测下丘脑胆囊收缩素-8(CCK-8)含量。结果与空白组比较,模型组大鼠海马组织中Glu含量显著升高(P<0.01),GABA含量显著降低(P<0.01),5-HT的阳性表达率显著降低(P<0.01),下丘脑组织中CCK-8的含量显著降低(P<0.01)。与模型组比较,清心神安方高、中剂量组Glu含量显著下降(P<0.01),GABA含量显著升高(P<0.01);清心神安方高、中、低剂量组均能显著提高海马组织中5-HT阳性表达率(P<0.01),提高失眠大鼠下丘脑组织中CCK-8的含量(P<0.01)。结论清心神安方具有降低失眠大鼠脑组织Glu含量,提高GABA、5-HT、CCK-8含量的作用。Objective To observe the effect of Qingxin Shenan decoction on neurotransmitters in brain of insomnia rats.Methods Male SD rats 10 were randomly selected as the blank group,the PCPA model group(300 mg·kg^-1),2 d after modeling were randomly divided into model group,Qingxin Shenan decoct ion high,medium,and low dose groups(32,16,8 g·kg^-1),and diazepam group(0.5 mg·kg^-1).The rats were intragastrically treated with correspondent drugs for 10 d.The contents of glutamate(Glu),gamma aminobutyric acid(GABA) in hippocampus of insomnia rats were detected by high-performance liquid phase chromatography(HPLC);the content of 5-hydroxytryptamine(5-HT) in hippocampal tissue was detected by immunohistochemical method;and expression of cholecystokinin 8(CCK-8)in the hypothalamus was detected by ELISA.Results Compared with the blank group,the content of Glu in hippocampus tissue of model group rats was significantly increased,the content of GABA was significantly decreased(P < 0.01),the positive expression rate of 5-HT was significantly reduced(P < 0.01),the content of CCK-8 was significantly decreased(P < 0.01).Compared with the model group,the content of Glu in hippocampus tissue of Qingxin Shenan decoction high,middle dose groups rats decreased significantly(P < 0.01);the content of GABA increased(P < 0.01);The content of 5-HT positive expression in hippocampus tissue and CCK-8 in hypothalamus treated with Qingxin Shenan decoction high,middle and low doses increased significantly,higher than that in the model group(P < 0.01).Conclusion Qingxin Shenan decoction can reduce the content of Glu in brain of insomnia rats,increase the contents of GABA,5-HT and CCK-8.

关 键 词：失眠 清心神安方 谷氨酸 Γ-氨基丁酸 5-羟色胺 胆囊收缩素-8 

分 类 号：R285.5[医药卫生—中药学]