生物样品前处理技术在药动学研究中的应用进展  

Progress on Application of Biological Sample Pretreatment Technology in Pharmacokinetic Research

作　　者：董金材 曾鳞粞 王曦 杨兴鑫 顾雯[1,2] 俞捷 DONG Jincai;ZENG Linxi;WANG Xi;YANG Xingxin;Gu Wen;YU Jie(College of Pharmaceutical Science,Yunnan University of Traditional Chinese Medicine,Kunming 650500 Yunnan,China;Kunming Key Laboratory for Metabolic Diseases Prevention and Treatment by Chinese Medicine,Kunming 650500 Yunnan,China)

机构地区：[1]云南中医学院中药学院,云南昆明650500 [2]昆明市代谢性疾病中医药防治重点实验室,云南昆明650500

出　　处：《中药新药与临床药理》2018年第1期110-117,共8页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金(81660596;81460623);云南省应用基础研究项目(2016FD050;2014FA035;2014FZ083);云南省中青年学术和技术带头人后备人才(2015HB053);云南省高校南药研究协同创新中心(30270100500)

摘　　要：生物样品前处理技术是药动学研究过程中最重要、最困难和最繁琐的步骤,选择合适的样品前处理技术是进行药动学研究的关键环节。本文综述了近年来药动学研究中广泛使用的样品前处理技术及其特点,包括蛋白沉淀、液液萃取、液相微萃取、固相萃取、固相微萃取、微透析、分子印迹、柱切换、超临界流体萃取。目前,样品前处理的新方法及新技术不断出现,而且正向着简便化、自动化、超灵敏化以及样品、试剂微量化发展。Technology for biological sample pretreatment is the most important,difficult and tedious procedure in pharmacokinetic research.The selection of appropriate technology of sample pretreatment plays a major part in the pharmacokinetic study.In this paper,the techniques of sample pretreatment widely-used in pharmacokinetic studies in recent years are reviewed with the future perspectives,including protein precipitation,liquid-liquid extraction,liquid phase microextraction,solid phase extraction,solid phase microextraction,microdialysis,molecular imprinting,column switching and critical fluid extraction.At present,the new methods and technologies of sample pretreatment are constantly emerging,which are developing towards simplification,automation,hypersensitization and miniaturization of sample sizes and reagent dosages.

关 键 词：生物样品 前处理技术 药动学 

分 类 号：R284.1[医药卫生—中药学]