Microchip–CE技术用于糖化血红蛋白的分离研究  

Analysis of Glycosylation Hemoglobin by Microfl uidic Chip – Capillary Electrophoresis

作　　者：郭文鹏[1] 唐丽 尚艳霞 潘力弢[1] 戎志斌[4] GUO Wen-peng;TANG Li;SHANG Yan-xia;PAN Li-tao;RONG Zhi-bin(The First Affiliated Hospital of Shenzhen University (Shenzhen Second People's Hospital),Guangdong Shenzhen 518035;The University of Hong Kong,Hong Kong SAR 999077;Dengfeng Environmental Protection Bureau,Henan Dengfeng 452470;Guang Anmen Hospital,China Academy of Chinese Medical Science,Beijing 100053)

机构地区：[1]深圳大学第一附属医院,深圳市第二人民医院,广东深圳518035 [2]香港大学,中国香港特别行政区999077 [3]登封市环境保护局,河南登封452470 [4]中国中医科学院广安门医院,北京100053

出　　处：《深圳中西医结合杂志》2018年第24期1-3,共3页Shenzhen Journal of Integrated Traditional Chinese and Western Medicine

基　　金：国家自然科学基金项目资助课题(81301739);广东省科技厅项目资助课题(2015A020214002);深圳市科技创新委员会资助项目(JCYJ20170306091043130)

摘　　要：目的:探讨基于微流控芯片–毛细管电泳(Microchip–CE)平台的糖化血红蛋白(HbA1c)快速分离方法研究及其临床应用价值。方法:利用Microchip–CE技术平台和糖化血红蛋白415 nm的特异性吸收峰,检测糖化血红蛋白在健康人群及高危人群中的水平。结果:从全血中成功分离出HbA1c,精确度和准确度均进行优化。结论:Microchip–CE法具有用量少、特异性好、精确度和准确度高等优点,可以有效检测健康人群及高危人群中的HbA1c水平,为临床提供一种新的检测方法。Objective To study the application value of the microfluidic chip-capillary electrophoresis in detecting the glycosylation hemoglobin level. Methods By utilizing the home-made microfl uidic chip-capillary electrophoresis device and the specifi c adsorption peak(415 nm), the level of HbA1 c in the healthy and high risk people was detected. Results The HbA1 c was effectively separated from the whole blood with the optional precision and accuracy. Conclusion The microfl uidic chip – capillary electrophoresis device is characterized by lower sample usage, specifi city, high precision and accuracy. It provides a complementary method in clinic.

关 键 词：微流控芯片 糖化血红蛋白 毛细管电泳检测 糖尿病诊断 

分 类 号：O658.9[理学—分析化学]