200例CDHR3基因单核苷酸多态性与人鼻病毒所致婴幼儿下呼吸道感染的关系  

Association of single nucleotide polymorphism of CDHR3 gene with lower respiratory tract rhinovirus infection in children:analysis of 200 cases

作　　者：李慧[1] 任洛[1] 张瑶 余漪漪 高钰 龙鑫 刘恩梅[2] LI Hui;REN Luo;ZHANG Yao;YU Yiyi;GAO Yu;LONG Xin;LIU Enmei(Institute of Pediatrics,Key Laboratory of Child Development and Disorders of Ministry of Education,China International Science and Technology Cooperation Base of Child Develop ment and Critical Disorders,Chongqing Key Laboratory of Pediatrics,Children’s Hospital of Chongqing Medical University,Chongqing,400014;Department of Respiratory Medicine,Children’s Hospital of Chongqing Medical University,Chongqing,401122,China)

机构地区：[1]重庆医科大学附属儿童医院儿科研究所,儿童发育疾病研究教育部重点实验室,儿童发育重大疾病国家国际科技合作基地,儿童感染免疫重庆市重点实验室,重庆400014 [2]重庆医科大学附属儿童医院呼吸中心,重庆401122

出　　处：《第三军医大学学报》2019年第4期371-375,共5页Journal of Third Military Medical University

摘　　要：目的探讨CDHR3基因单核苷酸多态性与人鼻病毒(human rhinoviruses,HRV)所致儿童急性下呼吸道感染的关系。方法收集2012年1月至2014年11月因HRV所致急性下呼吸道感染的患儿鼻咽抽吸物,提取RNA逆转录后,采用PCR方法扩增CDHR3基因片段,测序后进行rs6967330位点基因分型。利用PCR的方法扩增HRV的VP4基因进行测序分型,实时荧光定量PCR方法进行HRV载量测定,并回顾性分析rs6967330位点与患儿临床特征的相关性。结果共纳入200例患儿,其中AA/AG基因型30例(15. 0%),GG基因型170例(85. 0%)。HRV所致急性下呼吸道感染患儿中,AA/AG基因型与GG基因型相比更容易出现喘息症状(86. 7%vs 57. 1%,P=0. 002),而且更易致重症肺炎(30. 0%vs 11. 2%,P=0. 018),一级亲属也更多具有特应性疾病病史(16. 7%vs 5. 88%,P=0. 039)。Logistic回归分析发现rs6967330 A是HRV感染患儿喘息(OR:4. 585,95%CI:1. 446～14. 541,P=0. 010)及发展为重症病例(OR:3. 379,95%CI:1. 009～11. 315,P=0. 048)的危险因素。而HRV病毒亚型及载量在不同基因型间差异没有统计学意义。结论 CDHR3基因单核苷酸多态性位点rs6967330可能与HRV感染后的重症及喘息相关。Objective To explore the association of single nucleotide polymorphism( SNP) at rs6967330 of cadherin-associated family member 3( CDHR3) gene with acute respiratory tract infection caused by human rhinoviruses( HRVs) in children.Methods From January,2012 to November,2014,we collected specimens of nasopharyngeal aspirates from 200 children with acute lower respiratory tract infection caused by HRVs.CDHR3 gene fragment was amplified by PCR from the samples and sequenced for rs6967330 genotyping.The VP4 gene of HRV was amplified with PCR for subsequent sequencing,and realtime fluorescent quantitative PCR was used to estimate the virus load.The correlation between rs6967330 genotypes and the clinical features of the children was analyzed.Results Of the 200 children with acute lower respiratory tract infection caused by HRVs,30( 15.0%) had AA/AG genotype and 170( 85.0%) had GG genotype of rs6967330.The children with AA/AG genotype were more likely to have wheezing symptoms than those with GG genotype( 86.7% vs 57.1%,P = 0.002),and were also more susceptible to severe pneumonia( 30.0% vs 11.2%,P = 0.018);the first-degree relatives of the children with AA/AG genotype were also more likely to have a history of atopic diseases( 16.7% vs 5.88%,P = 0.039).Logistic regression analysis revealed that rs6967330 A was a risk factor for wheezing in children with HRV infection( OR: 4.585,95% CI: 1.446 ~ 14.541,P = 0.010) and also for progression into severe cases( OR: 3.379,95% CI:1.009 ~ 11.315,P = 0.048).No significant differences in HRV subtypes or viral loads were found between the children with different genotypes of rs6967330.Conclusion The SNP of CDHR3 gene at rs6967330 may be associated with severe cases and wheezing after lower respiratory tract infection with HRV in children.

关 键 词：CDHR3基因 单核苷酸 多态性 人鼻病毒 

分 类 号：R181.32[医药卫生—流行病学] R394.1[医药卫生—公共卫生与预防医学]