红花文殊兰的离体培养及快速繁殖  

In Vitro Culture and Rapid Propagation of Crinum amabile

作　　者：黄碧兰[1] 李志英 徐立[1] Huang Bilan;Li Zhiying;Xu Li(Institute of Tropical Crop Genetic Resources,Chinese Academy of Tropical Agricultural Sciences,Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China,and Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation, Danzhou,571737)

机构地区：[1]中国热带农业科学院热带作物品种资源研究所农业部热带作物种质资源利用重点开放华南作物基因资源与种质创制重点实验室海南省热带作物种质资源遗传改良与创新重点实验室,儋州571737

出　　处：《分子植物育种》2019年第3期928-933,共6页Molecular Plant Breeding

基　　金：物种资源保护项目(B650);中央级科研院所基本科研业务费专项(1630032018010)共同资助

摘　　要：以红花文殊兰的鳞茎为外植体,研究不同培养基对其腋芽启动、不定芽增殖及生根培养的影响。研究表明,腋芽启动的最适培养基为5.0 mg/L MS+6-BA+0.2 mg/L NAA+活性炭1 g/L,腋芽的诱导萌发率为88%;不定芽增殖的最适培养基为MS+2.0 mg/L 6-BA+0.5 mg/L TDZ+0.1 mg/L NAA+活性炭0.5 g/L,40 d的增殖系数可达7.16;壮苗培养基为1/2 MS+0.5 mg/L 6-BA+0.1 mg/L NAA+10%椰子水(CW)+活性炭0.5 g/L;生根的最适培养基为MS+0.5 mg/L NAA+0.5 mg/L IBA+活性炭0.5 g/L,生根率达100%,根系发达,植株生长健壮。生根苗经一段时间炼苗后,移栽到基质为树皮∶椰糠∶河砂=1∶1∶1的苗钵上,红花文殊兰的假植成活率达95%。该研究建立了红花文殊兰的快繁体系,为其工厂化生产提供技术支持。Using Crinum amabile bulbus as explants,we studied the effects of different mediums on axillary bud induction,proliferation and rooting.The results showed that the optimal medium for axillary bud induction was MS+5.0 mg/L 6-BA+0.1 mg/L NAA+I g/L active carbon,with an induction rate of 88%.The optimal proliferation medium was MS+2.0 mg/L 6-BA+0.5 mg/L TDZ+0.1 mg/L NAA+0.5 g/L active carbon,the propagation coefficie- nt was 7.16 every 40 days.The suitable medium for bud subculture was 1/2 MS+0.5 mg/L 6-BA+0.1 mg/L NAA+ 10%coconut water+0.5 g/L active carbon.The optimal rooting medium was MS+0.5 mg/L NAA+0.5 mg/L IBA+ 0.5 g/L active carbon,with rooting rate as high as 100%.The roots developed well and the plantlets grew vigorously.The plantlets with acclimatization were transplanted to bark:coconut bran:river sand=1:1:1 seeding bowl.The plantlets were successfully acclimatized and planted with a survival rate of 95%.The study established a fast propagation system for Crinum amabile and provided technical support for its factory production.

关 键 词：文殊兰 鳞茎 离体培养 

分 类 号：S682.29[农业科学—观赏园艺]