水溶性阳离子柱[5]芳烃还原石墨烯复合物在荧光检测左旋肉碱中的应用研究  

Application Research on the Fluorescence Detection of L-Carnitine by Water-soluble Cationic Pillar[5]arene-Reduce Graphene Composite

作　　者：谭晓平[1] 石洪凡 朱乾华[1] 罗莎莎 张庭璎 杨阳 TAN Xiao-ping;SHI Hong-fan;ZHU Qian-hua;LUO Sha-sha;ZHANG Ting-ying;YANG Yang(School of Chemistry and Chemical Engineering,Yangtze Normal University,Fuling 408100,China;Yunnan Institute for Food and Drug Supervision and Inspection,Kunming 650000,China)

机构地区：[1]长江师范学院化学化工学院,重庆涪陵408100 [2]云南省食品药品监督检验研究院,云南昆明650000

出　　处：《化学试剂》2019年第2期153-157,共5页Chemical Reagents

基　　金：重庆市教委科学技术研究项目(KJ1712298);重庆市科委基础科学基础前沿项目(cstc2017jcyjAX0031);校级创新平台项目(FLKW2017AAA1024)

摘　　要：采用湿化学还原法,在还原氧化石墨烯表面引入水溶性大环主体超分子柱[5]芳烃(CP5),形成复合物CP5-RGO。采用FTIR、XPS对复合物CP5-RGO进行表征。使用罗丹明123(R123)作为探针分子,CP5-RGO作为受体来构建荧光传感器,以此来检测左旋肉碱,其线性范围为0. 1～2. 0μmol/L和2. 0～25. 0μmol/L,检出限为0. 034μmol/L(S/N=3)。以牛奶为实际样品,采用加标回收方法探讨了R123@CP5-RGO传感器的实用性,其回收率为91. 0%～100. 2%,相对标准偏差(RSD)为2. 0%～2. 6%。所构建的荧光传感平台R123@CP5-RGO具有快速、灵敏、简便等优点,有望应用于实际样品的检测。A wet-chemistry reduction method was used to introduce a water-soluble cationic pillar[5]arene( CP5) onto the graphene surface to form composite CP5-RGO.The CP5-RGO was characterized by FTIR and XPS.A novel competitive fluorescence sensing platform towards L-carnitine was developed based on the receptor CP5-RGO and probe R123.Low detection limit of 0. 034μmol/L( S/N= 3) and linear response ranges of 0. 1~2. 0 μmol/L and 2. 0~25. 0 μmol/L for L-carnitine were obtained.It was successfully utilized to detect L-carnitine in milk samples and the recoveries were in the range of 91. 0% ~ 100. 2% and RSD were in the range of 2. 0% ~ 2. 6%.The fluorescent sensor platform based on R123@ CP5-RGO holds the advantages of rapidness,simplicity,and high sensitivity,which is expected for the detection of practical samples.

关 键 词：芳烃 还原氧化石墨烯 罗丹明123 左旋肉碱 

分 类 号：O652.7[理学—分析化学]