铁皮石斛提取物对DSS诱导的溃疡性结肠炎小鼠的抗氧化及抗炎作用  

Antioxidant and anti-inflammatory effects of Dendrobium officinale extract on DSS-induced ulcerative colitis model of mice

作　　者：刘志龙[1] 赵佩[2] 高进贤[2] 于静[2] 袁继勇[2] 吴树金[2] 呼延丽[2] 葛斌[2] 石磊[2] LIU Zhi-long;ZHAO Pei;GA0 Jin-xian;YU Jing;YUAN Ji-yong;WU Shu-jin;HU Yan-li;GE Bin;SHI Lei(Department of AnesthesiologyGansu Provincial Hospital,Lanzhou 730000,China;Department of Pharmacy,Gansu Provincial Hospital,Lanzhou 730000,China)

机构地区：[1]甘肃省人民医院麻醉科,兰州730000 [2]甘肃省人民医院药剂科,兰州730000

出　　处：《中国新药杂志》2019年第2期214-220,共7页Chinese Journal of New Drugs

基　　金：甘肃省自然科学基金资助项目(1606RJZA138)

摘　　要：目的:观察铁皮石斛提取物对葡聚糖硫酸钠(DSS)诱导的溃疡性结肠炎小鼠抗氧化、抗炎能力的影响。初步探讨铁皮石斛提取物治疗溃疡性结肠炎的作用机制。方法:将小鼠随机分为正常对照组、DSS模型组、5-氨基水杨酸(5-ASA)阳性药物对照组、铁皮石斛提取物低、中、高(200,400,800 mg·kg-1)剂量组,除正常对照组外,其余各组采用4%的DSS自由饮用建立小鼠溃疡性结肠炎模型。5-ASA阳性药物对照组和铁皮石斛提取物治疗组在造模d 1开始灌胃(ig)给药,连续给药10 d后处死各组小鼠,留取结肠组织,对各组小鼠结肠组织进行大体与组织病理学损伤评分。运用蛋白免疫印迹技术(Western Blot)检测小鼠结肠组织中c-Jun,c-Fos的表达,酶联免疫法(ELISA)检测结肠组织中丙二醛(MDA)、诱导型一氧化氮合酶(i NOS)、NO、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽(GSH)、超氧化物歧化酶(SOD)、总的一氧化氮合酶(TNOS)的含量。同时,眼眶取血,收集血清,ELISA检测血清中干扰素IFN-γ,TNF-α的含量。结果:与正常对照组比较,DSS模型组小鼠结肠大体评分与组织病理学损伤评分显著增加(P <0. 01)。与模型组比较,铁皮石斛提取物不同剂量组小鼠结肠组织大体评分和组织病理学损伤评分降低(P <0. 05或P <0. 01);血清中干扰素(IFN-γ,TNF-α)的含量明显降低(P <0. 05);结肠组织中c-Jun,c-Fos蛋白表达显著降低(P <0. 05);结肠组织中MDA,i NOS,NO,TNOS含量显著降低(P <0. 01或P <0. 05);而结肠组织中GSH-Px,GSH,SOD含量明显升高(P <0. 01)。结论:研究表明铁皮石斛提取物具有治疗DSS诱导的小鼠溃疡性结肠炎的作用,其作用机制之一是通过清除自由基、修复氧化损伤和提高细胞抗氧化能力,重新建立氧化还原平衡,同时,抑制炎症因子IFN-γ,TNF-α的释放和c-Jun,c-Fos的高表达,进而抑制结肠炎症。Objective:To observe the anti-oxidation and anti-inflammatory effects of Dendrobium officinale extract on dextran sodium sulfate (DSS)-induced ulcerative colitis in mice,and preliminarily disccuss the therapeutie mechanism of Dendrobium officinale extract on ulcerative colitis.Methods:Mice were randomly divided into normal control group,DSS model group,5-ASA treatment group,Dendrobium officinale extract low-,middle-,and high-dose (200,400,800 mg·kg^-1)treatment group.Except the normal control group,all the other groups were treated with 4%DSS to induce the ulcerative colitis model.From the first day of modeling,5-ASA and different concentrations of Dendrobium officinale were given by gavage for 10 days continuously.The mice were killed after drug treatment for 10 days,and the colon was taken.The colonic injury and inflammation were assessed by macroscopic and microscopic damage scores.The expression levels of c-Jun and c-Fos in colon were measured by Western blot, while the levels of MDA,iNOS,NO,GSH-Px,GSH,SOD and TNOS were measured by ELISA.Meanwile,the serum was collected to detect the levels of IFN-γ,and TNF-α by ELISA.Results:As compared with the normal control group,the colonic injuryand pathological scores in the model group were significantly higher (P <0.01 ). In comparison with the model group,Dendrobium officinale extract of different concentrations reduced the colonic injury and pathological scores (P <0.05 or P <0.01 ).At the same time,the concentrations of IFN-γ,and TNF-α in serum were decreased (P <0.05);c-Jun and c-Fos protein expressions in the colon tissue were also reduced significantly (P <0.05);the concentrations of MDA,iNOS,NO,TNOS in the colon tissue were decreased significantly (P <0.01 or P <0.05);while the concentrations of GSH-Px,GSH,and SOD were increased obviously (P <0.01 ).Conclusion :Dendrobium officinale extract has a curative effect on UC mice induced by DSS.The mechanism is to scavenge free radicals and increase the anti-oxidative capacity of cells in a degree,and re-establi

关 键 词：铁皮石斛提取物 BALB/C小鼠 溃疡性结肠炎 抗氧化 抗炎 

分 类 号：R285.5[医药卫生—中药学] R967[医药卫生—中医学]