美洲大蠊microRNA的初步研究  

Preliminary Research of microRNA in Periplaneta americana

作　　者：宝钲 杨茗羽 张修月[1] 岳碧松[1,2,3] 范振鑫 BAO Zheng;YANG Mingyu;ZHANG Xiuyue;YUE Bisong;FAN Zhenxin(Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education,College of Life Sciences,Sichuan University,Chengdu 610065,China;Sichuan Engineering Research Center for Medicinal Animals,Xichang,Sichuan Province 615000,China;Sichuan Key Laboratory for Medicinal American Cockroach,Chengdu 610031,China)

机构地区：[1]生物资源与生态环境教育部重点实验室四川大学生命科学学院,成都610065 [2]四川药用动物工程技术研究中心,四川西昌615000 [3]药用美洲大蠊四川省重点实验室,成都610031

出　　处：《四川动物》2019年第1期47-55,共9页Sichuan Journal of Zoology

基　　金：好医生助研项目

摘　　要：美洲大蠊Periplaneta americana是一种世界性的卫生害虫,但其提取物有较高的药用价值。本研究对饲养的雌雄美洲大蠊成虫进行microRNA测序,分别在雄性和雌性中得到12 155 616条和9 847 263条序列。序列长度主要为18～23 nt,且在22 nt和29 nt处有2个峰值。将所得序列与数据库(NCBI、Rfam)进行比对注释,最终在雄性成虫中鉴定到57种已知的microRNA和152种潜在的新microRNA,在雌性成虫中鉴定到53种已知的microRNA和94种潜在的新microRNA。差异表达分析发现只有一种microRNA:miR-750在雌雄之间差异表达,其在雌虫中表达量显著高于雄虫。本研究首次在基因组水平研究了美洲大蠊microRNA的组成并对其功能进行了预测,为其后续研究奠定了基础。American cockroach( Periplaneta americana) is known as one of the public health pests worldwide.Meanwhile,its extracts are valuable for medical use.In this study,sequencing of microRNAs of the reared cockroaches was carried out.A total of 12 155 616 and 9 847 263 sequences were obtained from the male and female individuals,respectively.The length of sequences mainly ranged from 18 nt to 23 nt with a bimodal distribution at 22 nt and 29 nt.Annotation of these clean reads were performed according to databases of Rfam and NCBI by Blastn.Finally,57 known microRNAs and 152 novel microRNAs were identified from the male group,while 53 known microRNAs and 94 novel microRNAs were identified from the female group.Differential expression analysis indicated that only one microRNA( miR-750) was significantly upregulated in female P.americana compared to that of the male.This is the first study concerning on the composition and function of microRNAs in P.americana at genomic level,and thus lay a foundation for further study on P.americana.

关 键 词：美洲大蠊 MICRORNA 差异表达 

分 类 号：Q78[生物学—分子生物学] Q915.819.7[天文地球—古生物学与地层学]